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beta Tubulin 3/ Tuj1 antibody

Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85469
Anti-beta Tubulin 3/ Tuj1 antibody used in Western Blot (WB). GTX85469
Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85469
Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85469

Cat No. GTX85469

Host

Chicken

Clonality

Polyclonal

Isotype

IgY

Application

WB, ICC/IF, IHC

Reactivity

Human, Mouse, Rat
Package
50 μg ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:10000-1:20000
ICC/IF Assay dependent
IHC 1:2000-1:5000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Product Note

tubulin, beta 3

PROPERTIES

Form

Liquid

Buffer

PBS + sodium azide (0.02%, w/v)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

100 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the β-Tubulin 3 gene product, but are shared between the human (NP_AAL28094, NCBI) and rat (AAM28438, NCBI) protein sequences.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10629222

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tubulin beta 3 class III , CDCBM , CDCBM1 , CFEOM3 , CFEOM3A , FEOM3 , TUBB4 , beta-4

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]

Database

Research Area

DATA IMAGES

Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85469

GTX85469 ICC/IF Image

ICC/IF analysis of paraformaldehyde-fixed (4%) neonatal mouse brain cultures using GTX85469 beta Tubulin 3 / Tuj1 antibody.
Green : Primary antibody
RED : GFAP antibody
BLUE : DAPI Dilution : 1:1000

Anti-beta Tubulin 3/ Tuj1 antibody used in Western Blot (WB). GTX85469

GTX85469 WB Image

WB analysis of adult mouse brain lysate using GTX85469 beta Tubulin 3 / Tuj1 antibody.
Dilution : 1:3000

Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85469

GTX85469 ICC/IF Image

ICC/IF analysis of paraformaldehyde-fixed (4%) SpH64 cells using GTX85469 beta Tubulin 3 / Tuj1 antibody.
Green : Primary antibody
RED : Actin antibody
BLUE : DAPI Dilution : 1:1000

Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85469

GTX85469 ICC/IF Image

ICC/IF analysis of paraformaldehyde-fixed (4%) SpH64 cells using GTX85469 beta Tubulin 3 / Tuj1 antibody.
Green : Primary antibody
RED : Actin antibody
BLUE : DAPI Dilution : 1:1000

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 289