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beta Tubulin 3/ Tuj1 antibody

Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85469
Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85469
Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85469
Anti-beta Tubulin 3/ Tuj1 antibody used in Western Blot (WB). GTX85469
Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85469
Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85469
Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85469
Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85469
Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85469

Cat. No. GTX85469

Reference 12

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB, ICC/IF, IHC

Reactivity

 Human, Mouse, Rat
Package
50 μg ($399)
See all beta Tubulin 3/ Tuj1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:10000-1:20000
ICC/IF Assay dependent
IHC 1:2000-1:5000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Product Note

tubulin, beta 3

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

100 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the β-Tubulin 3 gene product, but are shared between the human (NP_AAL28094, NCBI) and rat (AAM28438, NCBI) protein sequences.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10629222

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tubulin beta 3 class III , CDCBM , CDCBM1 , CFEOM3 , CFEOM3A , FEOM3 , TUBB4 , beta-4

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]

Database

Research Area

DATA IMAGES

Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85469

GTX85469 ICC/IF Image

ICC/IF analysis of paraformaldehyde-fixed (4%) SpH64 cells using GTX85469 beta Tubulin 3 / Tuj1 antibody.
Green : Primary antibody
RED : Actin antibody
BLUE : DAPI Dilution : 1:1000

Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85469

GTX85469 ICC/IF Image

ICC/IF analysis of paraformaldehyde-fixed (4%) neonatal mouse brain cultures using GTX85469 beta Tubulin 3 / Tuj1 antibody.
Green : Primary antibody
RED : GFAP antibody
BLUE : DAPI Dilution : 1:1000

Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85469

GTX85469 ICC/IF Image

ICC/IF analysis of paraformaldehyde-fixed (4%) SpH64 cells using GTX85469 beta Tubulin 3 / Tuj1 antibody.
Green : Primary antibody
RED : Actin antibody
BLUE : DAPI Dilution : 1:1000

Anti-beta Tubulin 3/ Tuj1 antibody used in Western Blot (WB). GTX85469

GTX85469 WB Image

WB analysis of adult mouse brain lysate using GTX85469 beta Tubulin 3 / Tuj1 antibody.
Dilution : 1:3000

Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85469

GTX85469 ICC/IF Image

The data was published in the journal Front Neurosci in 2018.PMID: 29515358

Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85469

GTX85469 ICC/IF Image

The data was published in the journal Cell Rep in 2018.PMID: 30089262

Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85469

GTX85469 ICC/IF Image

The data was published in the journal Front Neurosci in 2018.PMID: 29515358

Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85469

GTX85469 ICC/IF Image

The data was published in the journal Front Neurosci in 2018.PMID: 29515358

Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85469

GTX85469 ICC/IF Image

The data was published in the journal Front Neurosci in 2018.PMID: 29515358

REFERENCE

Application Reference

O'Brien BS et al. J Virol 2023; 97 (10) : e0069623 Neutralizing antibodies with neurotropic factor treatment maintain neurodevelopmental gene expression upon exposure to human cytomegalovirus.
Vahsen BF et al. Sci Rep 2022; 12 (1) : 12606 Human iPSC co-culture model to investigate the interaction between microglia and motor neurons.
Zhang X et al. Cells 2022; 11 (23) VDR Regulates BNP Promoting Neurite Growth and Survival of Cochlear Spiral Ganglion Neurons through cGMP-PKG Signaling Pathway.
Wang T et al. Nat Commun 2022; 13 (1) : 5584 Intracellular energy controls dynamics of stress-induced ribonucleoprotein granules.
Benjamin S O'Brien et al. iScience 2022; 25 (4) : 104098 Downregulation of neurodevelopmental gene expression in iPSC-derived cerebral organoids upon infection by human cytomegalovirus.
Application : ICC/IF
Reactivity : Human
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REVIEW

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Application Reference
O'Brien BS et al. J Virol 2023; 97 (10):e0069623 Neutralizing antibodies with neurotropic factor treatment maintain neurodevelopmental gene expression upon exposure to human cytomegalovirus.
Vahsen BF et al. Sci Rep 2022; 12 (1):12606 Human iPSC co-culture model to investigate the interaction between microglia and motor neurons.
Zhang X et al. Cells 2022; 11 (23) VDR Regulates BNP Promoting Neurite Growth and Survival of Cochlear Spiral Ganglion Neurons through cGMP-PKG Signaling Pathway.
Wang T et al. Nat Commun 2022; 13 (1):5584 Intracellular energy controls dynamics of stress-induced ribonucleoprotein granules.
Benjamin S O'Brien et al. iScience 2022; 25 (4):104098 Downregulation of neurodevelopmental gene expression in iPSC-derived cerebral organoids upon infection by human cytomegalovirus.
Application : ICC/IF
Reactivity : Human
Ravi Kumar et al. Cereb Cortex Commun 2020; 1 (1):tgaa053 Astrocytic Regulation of Synchronous Bursting in Cortical Cultures: From Local to Global.
Application : ICC/IF
Reactivity : Mouse
Roy S et al. Nanomedicine 2020; 28:102220 Neurogenic tissue nanotransfection in the management of cutaneous diabetic polyneuropathy.
Application : ICC/IF
Reactivity : Mouse
Sison SL et al. J Virol 2019; 93 (17) Human Cytomegalovirus Disruption of Calcium Signaling in Neural Progenitor Cells and Organoids.
Seminary ER et al. Front Neurosci 2018; 12:86 Modeling Protein Aggregation and the Heat Shock Response in ALS iPSC-Derived Motor Neurons.
Santarriaga S et al. Mol Cell 2018; 71 (2):216-228.e7 SRCP1 Conveys Resistance to Polyglutamine Aggregation.
Application : ICC/IF
Hauser M et al. Cell Rep 2018; 24 (6):1512-1522 The Spectrin-Actin-Based Periodic Cytoskeleton as a Conserved Nanoscale Scaffold and Ruler of the Neural Stem Cell Lineage.
Application : ICC/IF
Reactivity : Rat
Patitucci TN et al. Hum Mol Genet. 2015; (Epub) SMN deficiency does not induce oxidative stress in SMA iPSC-derived astrocytes or motor neurons.
Application : ICC/IF
Reactivity : Human
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399