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beta Tubulin 4 antibody

Anti-beta Tubulin 4 antibody used in Western Blot (WB). GTX102095
Anti-beta Tubulin 4 antibody used in Western Blot (WB). GTX102095
Anti-beta Tubulin 4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102095
Anti-beta Tubulin 4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102095

Cat. No. GTX102095

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all beta Tubulin 4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

NIH-3T3 , 293T

Predict Reactivity

Bovine, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta Tubulin 4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952440

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tubulin beta 4A class IVa , DYT4 , TUBB4 , beta-5

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Database

Research Area

DATA IMAGES

Anti-beta Tubulin 4 antibody used in Western Blot (WB). GTX102095

GTX102095 WB Image

Sample(30 μg of whole cell lysate)
A:293T
7.5% SDS PAGE
GTX102095 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Tubulin 4 antibody used in Western Blot (WB). GTX102095

GTX102095 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX102095 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Tubulin 4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102095

GTX102095 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using beta Tubulin 4(GTX102095) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-beta Tubulin 4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102095

GTX102095 ICC/IF Image

beta Tubulin 4 antibody detects beta Tubulin 4 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Tubulin 4 stained by beta Tubulin 4 antibody (GTX102095) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Huang HS et al. Carcinogenesis 2015; 36 (11) : 1419-28 Mutagenic, surviving and tumorigenic effects of follicular fluid in the context of p53 loss: initiation of fimbria carcinogenesis.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169