APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000-1:3000 |
1:50-1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 0.5% BSA, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
recombinant Protein
Purification
Purified by antigen-affinity chromatography
Conjugation
Horseradish peroxidase(HRP)
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CDCBM6,CSCSC1,M40,OK/SWcl.56,TUBB,TUBB1,TUBB5,tubulin beta class I,beta Tubulin
Cellular Localization
Cytoplasm,Cytoskeleton
Background
This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014]
Database
Research Area
DATA IMAGES
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GTX34285 IHC-P Image
IHC-P analysis of human colon tissue using GTX34285 beta Tubulin I antibody [5G3] (HRP). Negative control (the lower left coner) was secondary antibody only. Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min) Dilution : 1:200
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GTX34285 IHC-P Image
IHC-P analysis of mouse testis tissue using GTX34285 beta Tubulin I antibody [5G3] (HRP). Negative control (the lower left coner) was secondary antibody only. Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min) Dilution : 1:200
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GTX34285 WB Image
WB analysis of HeLa cell lysate using GTX34285 beta Tubulin I antibody [5G3] (HRP).
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GTX34285 IHC-P Image
IHC-P analysis of rat liver tissue using GTX34285 beta Tubulin I antibody [5G3] (HRP). Negative control (the lower left coner) was secondary antibody only. Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min) Dilution : 1:200
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GTX34285 IHC-P Image
IHC-P analysis of human stomach tissue using GTX34285 beta Tubulin I antibody [5G3] (HRP). Negative control (the lower left coner) was secondary antibody only. Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min) Dilution : 1:200
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GTX34285 IHC-P Image
IHC-P analysis of human stomach cancer tissue using GTX34285 beta Tubulin I antibody [5G3] (HRP). Negative control (the lower left coner) was secondary antibody only. Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min) Dilution : 1:200
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GTX34285 IHC-P Image
IHC-P analysis of rat spleen tissue using GTX34285 beta Tubulin I antibody [5G3] (HRP). Negative control (the lower left coner) was secondary antibody only. Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min) Dilution : 1:200
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GTX34285 IHC-P Image
IHC-P analysis of rat kidney tissue using GTX34285 beta Tubulin I antibody [5G3] (HRP). Negative control (the lower left coner) was secondary antibody only. Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min) Dilution : 1:200
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GTX34285 IHC-P Image
IHC-P analysis of rat heart tissue using GTX34285 beta Tubulin I antibody [5G3] (HRP). Negative control (the lower left coner) was secondary antibody only. Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min) Dilution : 1:200
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GTX34285 IHC-P Image
IHC-P analysis of mouse kidney tissue using GTX34285 beta Tubulin I antibody [5G3] (HRP). Negative control (the lower left coner) was secondary antibody only. Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min) Dilution : 1:200
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GTX34285 IHC-P Image
IHC-P analysis of human liver tissue using GTX34285 beta Tubulin I antibody [5G3] (HRP). Negative control (the lower left coner) was secondary antibody only. Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min) Dilution : 1:200
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GTX34285 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX34285 beta Tubulin I antibody [5G3] (HRP). Negative control (the lower left coner) was secondary antibody only. Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min) Dilution : 1:200
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GTX34285 IHC-P Image
IHC-P analysis of rat lung tissue using GTX34285 beta Tubulin I antibody [5G3] (HRP). Negative control (the lower left coner) was secondary antibody only. Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min) Dilution : 1:200
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GTX34285 IHC-P Image
IHC-P analysis of human lung cancer tissue using GTX34285 beta Tubulin I antibody [5G3] (HRP). Negative control (the lower left coner) was secondary antibody only. Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min) Dilution : 1:200
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GTX34285 IHC-P Image
IHC-P analysis of mouse liver tissue using GTX34285 beta Tubulin I antibody [5G3] (HRP). Negative control (the lower left coner) was secondary antibody only. Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min) Dilution : 1:200
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GTX34285 IHC-P Image
IHC-P analysis of mouse lung tissue using GTX34285 beta Tubulin I antibody [5G3] (HRP). Negative control (the lower left coner) was secondary antibody only. Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min) Dilution : 1:200
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GTX34285 IHC-P Image
IHC-P analysis of mouse heart tissue using GTX34285 beta Tubulin I antibody [5G3] (HRP). Negative control (the lower left coner) was secondary antibody only. Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min) Dilution : 1:200
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GTX34285 IHC-P Image
IHC-P analysis of mouse spleen tissue using GTX34285 beta Tubulin I antibody [5G3] (HRP). Negative control (the lower left coner) was secondary antibody only. Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min) Dilution : 1:200
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REFERENCE
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REVIEW
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