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beta Tubulin I antibody [SAP.4G5]

Anti-beta Tubulin I antibody [SAP.4G5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11312
Anti-beta Tubulin I antibody [SAP.4G5] used in Western Blot (WB). GTX11312

Cat. No. GTX11312

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SAP.4G5

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP, ELISA

Reactivity

 Human, Mouse, Rat, Bovine, Dog, Hamster, Chicken, Xenopus
Package
100 μl ($409)
See all beta Tubulin I products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:20,000
ICC/IF Assay dependent
IHC-P Assay dependent
IP Assay dependent
ELISA Assay dependent

Note :

WB
using methanol/acetone-fixed chicken fibroblasts
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

chicken fibroblast

Product Note

Recognizes an epitope located in the C-terminal sequence of β-tubulin isotype I. No reactivity with other tubulin isotypes is observed.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

synthetic peptide corresponding to the C-terminal sequence of β-tubulin isotype I, conjugated to BSA.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_384213

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tubulin beta class I , CDCBM6 , CSCSC1 , M40 , OK/SW-cl.56 , TUBB1 , TUBB5

Cellular Localization

Cytoplasm, cytoskeleton

Background

The major building block of microtubules is tubulin. Tubulin exists as a heterodimer of two similar but not identical polypeptides, designated alpha and beta, that assembles into microtubules. For beta-tubulin, six evolutionarily conserved isotypes were identified (designated beta I - beta VI). The most complex pattern of isotype distribution in tissues is seen in the vertebrate beta-tubulins. In mammals and birds, betaI is constitutive and found in most tissues.

Database

Research Area

DATA IMAGES

Anti-beta Tubulin I antibody [SAP.4G5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11312

GTX11312 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX11312 beta Tubulin I antibody [SAP.4G5] at 1:5000 (green) with DAPI(blue).Cells were fixed and permeabilized with 4% PFA followed by 0.5% Triton X-100.

Anti-beta Tubulin I antibody [SAP.4G5] used in Western Blot (WB). GTX11312

GTX11312 WB Image

WB analysis of (1) chicken fibroblasts (2) mouse brain extract I (3) mouse brain extract II (4) rat brain (5) Jurkat (6) Daudi (7) SK-Mel-28 (8) G361 (9) HeLa using GTX11312 beta Tubulin I antibody [SAP.4G5] at 1:20000.

REFERENCE

Application Reference

Gumuscu B et al. Lab Chip 2020; 20 (1) : 64-73 Separation-encoded microparticles for single-cell western blotting.
Jung D et al. Sci Rep 2018; 8 (1) : 2487 Quinacrine upregulates p21/p27 independent of p53 through autophagy-mediated downregulation of p62-Skp2 axis in ovarian cancer.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 409