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beta Tubulin antibody [D66]

Anti-beta Tubulin antibody [D66] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11307
Anti-beta Tubulin antibody [D66] used in Western Blot (WB). GTX11307

Cat No. GTX11307

Host

Mouse

Clonality

Monoclonal

Clone Name

D66

Isotype

IgG1

Application

WB, ICC/IF, IP, ELISA

Reactivity

Human, Mouse, Rat, Rabbit, Bovine, Dog, Hamster, Chicken, Monkey, Chlamydomonas reinhardtii, Dinoflagellate, Sea Urchin
Package
100 μl ($389)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1.0 μg/ml
ICC/IF Assay dependent
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

PC12

Product Note

Recognizes all mouse brain β-tubulin isoforms (β and β′ subpopulations), and sea urchin β2-tubulin. The epitope recognized by the antibody is localized in the C-terminal region of β-tubulin (amino acids 427-432 in sea urchin β2-tubulin).

PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4, 1% BSA

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

~1? mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Sea Urchin

Immunogen

sea urchin (Lytechinus pictus) sperm axonemal proteins.

Purification

Purified immunoglobulin

Conjugation

Unconjugated

RRID

AB_372176

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tubulin beta class I , CDCBM6 , CSCSC1 , M40 , OK/SW-cl.56 , TUBB1 , TUBB5

Cellular Localization

Cytoplasm, cytoskeleton

Background

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.

Database

Research Area

DATA IMAGES

Anti-beta Tubulin antibody [D66] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11307

GTX11307 ICC/IF Image

ICC/IF analysis of SH-SY-5Y cells using GTX11307 beta Tubulin antibody [D66] at 0.5 μg/mL(red) with DAPI (blue). Cells were fixed and permeabilized with methanol followed by methanol:acetone.

Anti-beta Tubulin antibody [D66] used in Western Blot (WB). GTX11307

GTX11307 WB Image

WB analysis of (1) HeLa, (2) U87, (3) P19, (4) Neuro-2a, (5) PC-12, (6) COS-7, (7) CHO, (8) MDBK and (9) MDCK lysates using beta Tubulin antibody [D66] at of 1 μg/ml.

REFERENCE

REVIEW

beta Tubulin antibody [D66] Cat No. GTX11307

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016. PMID: 26840566
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cancer Med in 2019. PMID: 31274246
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cancer Med in 2019. PMID: 31274246
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 389