beta Tubulin antibody [D66]

Name: beta Tubulin antibody [D66]
Brand: GeneTex
SKU: GTX11307
Price: 439.00 USD
Rating: 5 (3 reviews)

Anti-beta Tubulin antibody [D66] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11307

Anti-beta Tubulin antibody [D66] used in Western Blot (WB). GTX11307

Cat. No. GTX11307

Reference 3

Host	Mouse
Clonality	Monoclonal
Clone Name	D66
Isotype	IgG1
Application	WB, ICC/IF, IP, ELISA
Reactivity	Human, Mouse, Rat, Rabbit, Bovine, Dog, Hamster, Chicken, Monkey, Chlamydomonas reinhardtii, Dinoflagellate, Sea Urchin
Package	100 μl ($439)

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	0.5-1.0 μg/ml
ICC/IF	Assay dependent
IP	Assay dependent
ELISA	Assay dependent

Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

PC12

Product Note

Recognizes all mouse brain β-tubulin isoforms (β and β′ subpopulations), and sea urchin β2-tubulin. The epitope recognized by the antibody is localized in the C-terminal region of β-tubulin (amino acids 427-432 in sea urchin β2-tubulin).

PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4, 1% BSA

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

~1? mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Sea Urchin

Immunogen

sea urchin (Lytechinus pictus) sperm axonemal proteins.

Purification

Purified immunoglobulin

Conjugation

Unconjugated

RRID

AB_372176

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tubulin beta class I , CDCBM6 , CSCSC1 , M40 , OK/SW-cl.56 , TUBB1 , TUBB5

Cellular Localization

Cytoplasm, cytoskeleton

Background

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.

Database

Gene ID: 203068 TUBB
UniProt: P07437 TUBB

Research Area

DATA IMAGES

GTX11307 ICC/IF Image

ICC/IF analysis of SH-SY-5Y cells using GTX11307 beta Tubulin antibody [D66] at 0.5 μg/mL(red) with DAPI (blue). Cells were fixed and permeabilized with methanol followed by methanol:acetone.

GTX11307 WB Image

WB analysis of (1) HeLa, (2) U87, (3) P19, (4) Neuro-2a, (5) PC-12, (6) COS-7, (7) CHO, (8) MDBK and (9) MDCK lysates using beta Tubulin antibody [D66] at of 1 μg/ml.

REFERENCE

Application Reference

Lee WJ et al. Cancer Med 2019; 8 (10) : 4821-4835 Melatonin promotes neuroblastoma cell differentiation by activating hyaluronan synthase 3-induced mitophagy.

Application : WB

Reactivity : Mouse

Lin JH et al. Cell Adh Migr 2019; : 1-18 Small G protein signalling modulator 2 (SGSM2) is involved in oestrogen receptor-positive breast cancer metastasis through enhancement of migratory cell adhesion via interaction with E-cadherin.

Application : ICC/IF

Reactivity : Human

Juenemann K et al. Neurotox Res 2011; 20 (2) : 120-33 Modulation of mutant huntingtin N-terminal cleavage and its effect on aggregation and cell death.

Application : WB

Reactivity : Human

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