GeneTex
United States (US)

beta Tubulin antibody

GTX101279_42018_20150715_IHC-P_R_2_19011506_431.jpg
GTX101279_42018_20150715_IHC-P_R_19011506_472.jpg
GTX101279_42018_20150306_WB_19011506_544.jpg
GTX101279_42018_20170119_WB_19011506_478.jpg
GTX101279_39645_IFA_19011506_294.jpg
GTX101279_IHC_19011506_248.jpg
GTX101279_39645_WB_sensitive_19011506_873.jpg
GTX101279_39645_WB_M_19011506_422.jpg
GTX101279_39645_IHC_19011506_847.jpg

Cat No. GTX101279

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat, Hamster
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A549 , H1299 , HCT116 , NIH-3T3 , *MCF7 , *Huh7

Predict Reactivity

Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta Tubulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

M40 antibody, MGC117247 antibody, MGC16435 antibody, OK/SW-cl.56 antibody, TUBB1 antibody, TUBB5 antibody, TUBB antibody, beta1-tubulin antibody, beta-4 tubulin antibody, tubulin beta-1 chain antibody, tubulin beta chain antibody, tubulin beta polypeptide antibody, "tubulin, beta polypeptide antibody", class I beta-tubulin antibody, beta Ib tubulin antibody, beta 5-tubulin antibody, tubulin beta-5 chain antibody, "tubulin, beta antibody"

Research Area

DATA IMAGES
GTX101279_42018_20150715_IHC-P_R_2_19011506_431.jpg

GTX101279 IHC-P Image

beta Tubulin antibody detects beta Tubulin protein at cytoplasm in rat spinal cord by immunohistochemical analysis.
Sample: Paraffin-embedded rat spinal cord.
beta Tubulin antibody (GTX101279) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101279_42018_20150715_IHC-P_R_19011506_472.jpg

GTX101279 IHC-P Image

beta Tubulin antibody detects beta Tubulin protein at cytoplasm in rat spleen by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
beta Tubulin antibody (GTX101279) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101279_42018_20150306_WB_19011506_544.jpg

GTX101279 WB Image

beta Tubulin antibody detects beta Tubulin protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin antibody (GTX101279) diluted at a dilution of 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101279_42018_20170119_WB_19011506_478.jpg

GTX101279 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin antibody (GTX101279) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101279_39645_IFA_19011506_294.jpg

GTX101279 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using beta Tubulin(GTX101279) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX101279_IHC_19011506_248.jpg

GTX101279 IHC-P Image

beta Tubulin antibody detects beta Tubulin protein at cytoplasm on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
beta Tubulin antibody (GTX101279) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101279_39645_WB_sensitive_19011506_873.jpg

GTX101279 WB Image

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
10% SDS PAGE
GTX101279 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101279_39645_WB_M_19011506_422.jpg

GTX101279 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX101279 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101279_39645_IHC_19011506_847.jpg

GTX101279 IHC-P Image

Immunohistochemical analysis of paraffin-embedded AGS xenograft, using beta Tubulin(GTX101279) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW

beta Tubulin antibody Cat No. GTX101279

Rating ( Average 4 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4 based on 2 users reviews)
Date : Anonymous submitted on 04-Jun-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells
Amount used : 20μg
Blocking : 5% skim milk in TBS, 25°C, 0.5Hr
Primary Antibody : 1:5000, 4°C, overnightHr
Date : Anonymous submitted on 04-Jun-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : MCF7 cells
Amount used : 20μg
Blocking : 5% skim milk in TBS, 25°C, 0.5Hr
Primary Antibody : 1:5000, 4°C, overnightHr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169