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United States (US)

beta Tubulin antibody

GTX101279_42018_20150715_IHC-P_R_2_19011506_431.jpg
GTX101279_42018_20150715_IHC-P_R_19011506_472.jpg
GTX101279_42018_20150306_WB_19011506_544.jpg
GTX101279_42018_20170119_WB_19011506_478.jpg
GTX101279_39645_IFA_19011506_294.jpg
GTX101279_IHC_19011506_248.jpg
GTX101279_39645_WB_sensitive_19011506_873.jpg
GTX101279_39645_WB_M_19011506_422.jpg
GTX101279_39645_IHC_19011506_847.jpg

Cat No. GTX101279

Reference 23
2
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat, Hamster
See all beta Tubulin products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A549 , H1299 , HCT116 , NIH-3T3 , *MCF7 , *Huh7

Predict Reactivity

Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta Tubulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

M40 antibody, MGC117247 antibody, MGC16435 antibody, OK/SW-cl.56 antibody, TUBB1 antibody, TUBB5 antibody, TUBB antibody, beta1-tubulin antibody, beta-4 tubulin antibody, tubulin beta-1 chain antibody, tubulin beta chain antibody, tubulin beta polypeptide antibody, "tubulin, beta polypeptide antibody", class I beta-tubulin antibody, beta Ib tubulin antibody, beta 5-tubulin antibody, tubulin beta-5 chain antibody, "tubulin, beta antibody"

Research Area

DATA IMAGES
GTX101279_42018_20150715_IHC-P_R_2_19011506_431.jpg

GTX101279 IHC-P Image

beta Tubulin antibody detects beta Tubulin protein at cytoplasm in rat spinal cord by immunohistochemical analysis.
Sample: Paraffin-embedded rat spinal cord.
beta Tubulin antibody (GTX101279) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX101279_42018_20150715_IHC-P_R_19011506_472.jpg

GTX101279 IHC-P Image

beta Tubulin antibody detects beta Tubulin protein at cytoplasm in rat spleen by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
beta Tubulin antibody (GTX101279) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX101279_42018_20150306_WB_19011506_544.jpg

GTX101279 WB Image

beta Tubulin antibody detects beta Tubulin protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin antibody (GTX101279) diluted at a dilution of 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX101279_42018_20170119_WB_19011506_478.jpg

GTX101279 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin antibody (GTX101279) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX101279_39645_IFA_19011506_294.jpg

GTX101279 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using beta Tubulin(GTX101279) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
GTX101279_IHC_19011506_248.jpg

GTX101279 IHC-P Image

beta Tubulin antibody detects beta Tubulin protein at cytoplasm on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
beta Tubulin antibody (GTX101279) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX101279_39645_WB_sensitive_19011506_873.jpg

GTX101279 WB Image

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
10% SDS PAGE
GTX101279 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX101279_39645_WB_M_19011506_422.jpg

GTX101279 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX101279 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX101279_39645_IHC_19011506_847.jpg

GTX101279 IHC-P Image

Immunohistochemical analysis of paraffin-embedded AGS xenograft, using beta Tubulin(GTX101279) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
REFERENCE

Application Reference

Pai VC et al. Oncogene 2018; (Epub ahead of print) ASPM promotes prostate cancer stemness and progression by augmenting Wnt-Dvl-3-β-catenin signaling.
Application : WB
Reactivity : Human
Pai VC et al. Oncogene 2018; (Epub ahead of print) ASPM promotes prostate cancer stemness and progression by augmenting Wnt-Dvl-3-β-catenin signaling.
Application : WB
Reactivity : Human
Wakae K et al. Sci Rep 2018; 8 (1):9745 Keratinocyte differentiation induces APOBEC3A, 3B, and mitochondrial DNA hypermutation.
Application : WB
Reactivity : Human
Lin M et al. Phytother Res 2018; (Epub ahead of print) Quercetin improves postpartum hypogalactia in milk-deficient mice via stimulating prolactin production in pituitary gland.
Application : WB
Reactivity : Mouse
Wang F et al. Mol Cancer Ther 2018; 17 (4):763-775 SKLB-23bb, A HDAC6-Selective Inhibitor, Exhibits Superior and Broad-Spectrum Antitumor Activity via Additionally Targeting Microtubules.
Application : WB
Reactivity : Human
Show More +
Submit a Reference
REVIEW

beta Tubulin antibody Cat No. GTX101279

Rating
( Average 4 based on 2 users reviews)
Application
Western Blot(WB)
( Average 4 based on 2 users reviews)
Date : Anonymous submitted on 04-Jun-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells
Amount used : 20μg
Blocking : 5% skim milk in TBS, 25°C, 0.5Hr
Primary Antibody : 1:5000, 4°C, overnightHr
Date : Anonymous submitted on 04-Jun-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : MCF7 cells
Amount used : 20μg
Blocking : 5% skim milk in TBS, 25°C, 0.5Hr
Primary Antibody : 1:5000, 4°C, overnightHr
Submit a Review
Application Reference
Pai VC et al. Oncogene 2018; (Epub ahead of print) ASPM promotes prostate cancer stemness and progression by augmenting Wnt-Dvl-3-β-catenin signaling.
Application : WB
Reactivity : Human
Pai VC et al. Oncogene 2018; (Epub ahead of print) ASPM promotes prostate cancer stemness and progression by augmenting Wnt-Dvl-3-β-catenin signaling.
Application : WB
Reactivity : Human
Wakae K et al. Sci Rep 2018; 8 (1):9745 Keratinocyte differentiation induces APOBEC3A, 3B, and mitochondrial DNA hypermutation.
Application : WB
Reactivity : Human
Lin M et al. Phytother Res 2018; (Epub ahead of print) Quercetin improves postpartum hypogalactia in milk-deficient mice via stimulating prolactin production in pituitary gland.
Application : WB
Reactivity : Mouse
Yoshimoto M et al. Oncology 2018; (Epub ahead of print) Abnormal Expression of PICT-1 and Its Codon 389 Polymorphism Is a Risk Factor for Human Endometrial Cancer.
Application : WB
Reactivity : Human
Wang F et al. Mol Cancer Ther 2018; 17 (4):763-775 SKLB-23bb, A HDAC6-Selective Inhibitor, Exhibits Superior and Broad-Spectrum Antitumor Activity via Additionally Targeting Microtubules.
Application : WB
Reactivity : Human
Yu-Lee LY et al. Cancer Res 2018; (Epub ahead of print) Osteoblast-secreted factors mediate dormancy of metastatic prostate cancer in the bone via activation of the TGFβRIII-p38MAPK-pS249/T252RB pathway.
Application : WB
Reactivity : Human
Her LS et al. Theranostics 2017; 7 (9):2452-2462 miR-196a Enhances Neuronal Morphology through Suppressing RANBP10 to Provide Neuroprotection in Huntington's Disease.
Application : ICC/IF
Reactivity : Mouse
Cheng JC et al. Sci Rep 2017; 7 (1):4448 Activation of hepatic stellate cells by the ubiquitin C-terminal hydrolase 1 protein secreted from hepatitis C virus-infected hepatocytes.
Application : WB
Reactivity : Rat
Chiang WC et al. Proc Natl Acad Sci U S A 2017; 114 (2):400-405 Achromatopsia mutations target sequential steps of ATF6 activation.
Application : WB
Reactivity : Human
Yoshimoto M et al. Mol Carcinog 2016; (Epub ahead of print) The Protein Interacting with Carboxyl Terminus-1 codon 389 polymorphism impairs protein interacting with carboxyl terminus-1 function and is a risk factor for uterine cervical cancer.
Albert JS et al. Orphanet J Rare Dis 2015; 10:27 Impaired osteoblast and osteoclast function characterize the osteoporosis of Snyder - Robinson syndrome.
Application : WB
Reactivity : Human
Hsiao WY et al. PLoS One 2015; 10 (12):e0145880 Dual-Specificity Phosphatase 4 Regulates STAT5 Protein Stability and Helper T Cell Polarization.
Application : WB
Reactivity : Human
Kohl S et al. Nat Genet 2015; 47 (7):757-65 Mutations in the unfolded protein response regulator ATF6 cause the cone dysfunction disorder achromatopsia.
Lin YJ et al. Biomedicine (Taipei) 2015; 5 (2):10 P-coumaric acid regulates exon 12 splicing of the ATP7B gene by modulating hnRNP A1 protein expressions.
Application : WB
Reactivity : Hamster
Murakami S et al. Neurochem Res 2015; 40 (6):1165-78 Long-Term Systemic Exposure to Rotenone Induces Central and Peripheral Pathology of Parkinson's Disease in Mice.
Application : IHC-P
Reactivity : Mouse
Lee YZ et al. Oncotarget 2014; 5 (15):6087-101 Discovery of selective inhibitors of Glutaminase-2, which inhibit mTORC1, activate autophagy and inhibit proliferation in cancer cells.
Application : WB
Reactivity : Human
Roy D et al. Cancer Metab 2014; 2:13 Loss of HSulf-1 promotes altered lipid metabolism in ovarian cancer.
Application : WB
Reactivity : Human
Lin YJ et al. Cell Biosci 2014; 4:40 Ring finger protein 39 genetic variants associate with HIV-1 plasma viral loads and its replication in cell culture.
Law CH et al. Toxicology 2013; 303:54-71 Hyaluronic acid-dependent protection in H9C2 cardiomyocytes: a cell model of heart ischemia-reperfusion injury and treatment.
Application : WB
Reactivity : Rat
Fan CY et al. Electrophoresis 2013; 34 (24):3305-14 Proteomic and redox-proteomic study on the role of glutathione reductase in human lung cancer cells.
Application : WB
Reactivity : Human
Lin ST et al. J Proteomics 2012; 75 (18):5822-47 Proteomic analysis of proteins responsible for the development of doxorubicin resistance in human uterine cancer cells.
Application : WB
Reactivity : Human
Cho CK et al. J Proteome Res 2010; 9 (7):3574-82 Amniotic fluid proteome analysis from Down syndrome pregnancies for biomarker discovery.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169