beta Tubulin antibody

Name: beta Tubulin antibody
Brand: GeneTex
SKU: GTX107175
Price: 399.00 USD
Rating: 5 (2 reviews)

Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX107175

Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175

Anti-beta Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107175

Cat. No. GTX107175

Reference 6

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, IP
Reactivity	Human, Mouse, Zebrafish
Package	100 μl ($399), 25 μl ($169)

See all beta Tubulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	Assay dependent
IHC-Fr	Assay dependent
IP	Assay dependent

Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

A431 , HepG2 , NIH-3T3

Predict Reactivity

Bovine, Cat, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta Tubulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952436

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tubulin beta 1 class VI

Background

Microtubules are involved in a wide variety of cellular processes, including mitosis, morphogenesis, platelet formation, and mobility of cilia and flagella. Circulating platelets carry a single marginal microtubule coil that is wound in 8 to 12 turns and is responsible for platelet shape. TUBB1 is the major beta-tubulin expressed in platelets and megakaryocytes and is required for optimal platelet assembly (Wang et al., 1986 [PubMed 3782288]; Schulze et al., 2004 [PubMed 15315966]).[supplied by OMIM]

Database

Gene ID: 81027 TUBB1
UniProt: Q9H4B7 TUBB1

Research Area

DATA IMAGES

GTX107175 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using beta Tubulin antibody (GTX107175) at 1:300 dilution.

GTX107175 WB Image

Sample (30 μg of whole cell lysate)
A: Adult zebrafish
7.5% SDS PAGE
GTX107175 diluted at 1:1000

GTX107175 WB Image

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
B:Hep G2 (GTX27900)
10% SDS PAGE
GTX107175 diluted at 1:1000

GTX107175 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX107175 diluted at 1:1000

GTX107175 ICC/IF Image

beta Tubulin antibody detects beta Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Tubulin protein stained by beta Tubulin antibody (GTX107175) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Anderson Tan et al. Cell Death Dis 2021; 12 (4) : 343 TFEB regulates pluripotency transcriptional network in mouse embryonic stem cells independent of autophagy-lysosomal biogenesis.

Application : WB, IP

Reactivity : Human

Heib T et al. Cell Rep 2021; 35 (6) : 109102 RhoA/Cdc42 signaling drives cytoplasmic maturation but not endomitosis in megakaryocytes.

Application : WB, IHC-Fr

Reactivity : Mouse

Cullmann K et al. Res Pract Thromb Haemost 2021; 5 (1) : 111-124 Forming megakaryocytes from murine-induced pluripotent stem cells by the inducible overexpression of supporting factors.

Moon S et al. EMBO Rep 2017; 18 (1) : 61-71 Phosphorylation by NLK inhibits YAP-14-3-3-interactions and induces its nuclear localization.

Application : WB

Reactivity : Human

Sprowl-Tanio S et al. Cancer Metab 2016; 4 : 20 Lactate/pyruvate transporter MCT-1 is a direct Wnt target that confers sensitivity to 3-bromopyruvate in colon cancer.

Application : WB

Reactivity : Human

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