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beta Tubulin antibody

Anti-beta Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107175
Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175
Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175

Cat No. GTX107175

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all beta Tubulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3

Predict Reactivity

Bovine, Cat, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta Tubulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tubulin beta 1 class VI

Background

Microtubules are involved in a wide variety of cellular processes, including mitosis, morphogenesis, platelet formation, and mobility of cilia and flagella. Circulating platelets carry a single marginal microtubule coil that is wound in 8 to 12 turns and is responsible for platelet shape. TUBB1 is the major beta-tubulin expressed in platelets and megakaryocytes and is required for optimal platelet assembly (Wang et al., 1986 [PubMed 3782288]; Schulze et al., 2004 [PubMed 15315966]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-beta Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107175

GTX107175 ICC/IF Image

beta Tubulin antibody detects beta Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Tubulin protein stained by beta Tubulin antibody (GTX107175) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175

GTX107175 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX107175 diluted at 1:1000

Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175

GTX107175 WB Image

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
B:Hep G2 (GTX27900)
10% SDS PAGE
GTX107175 diluted at 1:1000

REFERENCE

Application Reference

Moon S et al. EMBO Rep 2017; 18 (1) : 61-71 Phosphorylation by NLK inhibits YAP-14-3-3-interactions and induces its nuclear localization.
Application : WB
Reactivity : Human
Sprowl-Tanio S et al. Cancer Metab 2016; 4 : 20 Lactate/pyruvate transporter MCT-1 is a direct Wnt target that confers sensitivity to 3-bromopyruvate in colon cancer.
Application : WB
Reactivity : Human
Tsai BP et al. Open Biol 2014; 4 (11) : 140180 A novel Bcr-Abl-mTOR-eIF4A axis regulates IRES-mediated translation of LEF-1.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169