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beta Tubulin antibody

Anti-beta Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107175
Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175
Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175

Cat No. GTX107175

Reference 5
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all beta Tubulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3

Predict Reactivity

Bovine, Cat, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta Tubulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952436

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tubulin beta 1 class VI

Background

Microtubules are involved in a wide variety of cellular processes, including mitosis, morphogenesis, platelet formation, and mobility of cilia and flagella. Circulating platelets carry a single marginal microtubule coil that is wound in 8 to 12 turns and is responsible for platelet shape. TUBB1 is the major beta-tubulin expressed in platelets and megakaryocytes and is required for optimal platelet assembly (Wang et al., 1986 [PubMed 3782288]; Schulze et al., 2004 [PubMed 15315966]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-beta Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107175

GTX107175 ICC/IF Image

beta Tubulin antibody detects beta Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Tubulin protein stained by beta Tubulin antibody (GTX107175) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175

GTX107175 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX107175 diluted at 1:1000

Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175

GTX107175 WB Image

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
B:Hep G2 (GTX27900)
10% SDS PAGE
GTX107175 diluted at 1:1000

REFERENCE

Application Reference

Heib T et al. Cell Rep 2021; 35 (6) : 109102 RhoA/Cdc42 signaling drives cytoplasmic maturation but not endomitosis in megakaryocytes.
Application : WB, IHC-Fr
Reactivity : Mouse
Cullmann K et al. Res Pract Thromb Haemost 2021; 5 (1) : 111-124 Forming megakaryocytes from murine-induced pluripotent stem cells by the inducible overexpression of supporting factors.
Moon S et al. EMBO Rep 2017; 18 (1) : 61-71 Phosphorylation by NLK inhibits YAP-14-3-3-interactions and induces its nuclear localization.
Application : WB
Reactivity : Human
Sprowl-Tanio S et al. Cancer Metab 2016; 4 : 20 Lactate/pyruvate transporter MCT-1 is a direct Wnt target that confers sensitivity to 3-bromopyruvate in colon cancer.
Application : WB
Reactivity : Human
Tsai BP et al. Open Biol 2014; 4 (11) : 140180 A novel Bcr-Abl-mTOR-eIF4A axis regulates IRES-mediated translation of LEF-1.
Submit a Reference

REVIEW

beta Tubulin antibody Cat No. GTX107175

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cancer Metab in 2016.PMID: 27729975
Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Open Biol in 2014.PMID: 25392452
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169