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beta Tubulin antibody

Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX107175
Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175
Anti-beta Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107175
Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175
Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175

Cat. No. GTX107175

Reference 6
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all beta Tubulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC-Fr Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

A431 , HepG2 , NIH-3T3

Predict Reactivity

Bovine, Cat, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta Tubulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952436

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tubulin beta 1 class VI

Background

Microtubules are involved in a wide variety of cellular processes, including mitosis, morphogenesis, platelet formation, and mobility of cilia and flagella. Circulating platelets carry a single marginal microtubule coil that is wound in 8 to 12 turns and is responsible for platelet shape. TUBB1 is the major beta-tubulin expressed in platelets and megakaryocytes and is required for optimal platelet assembly (Wang et al., 1986 [PubMed 3782288]; Schulze et al., 2004 [PubMed 15315966]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX107175

GTX107175 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using beta Tubulin antibody (GTX107175) at 1:300 dilution.

Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175

GTX107175 WB Image

Sample (30 μg of whole cell lysate)
A: Adult zebrafish
7.5% SDS PAGE
GTX107175 diluted at 1:1000

Anti-beta Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107175

GTX107175 ICC/IF Image

beta Tubulin antibody detects beta Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Tubulin protein stained by beta Tubulin antibody (GTX107175) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175

GTX107175 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX107175 diluted at 1:1000

Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175

GTX107175 WB Image

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
B:Hep G2 (GTX27900)
10% SDS PAGE
GTX107175 diluted at 1:1000

REFERENCE

Application Reference

Anderson Tan et al. Cell Death Dis 2021; 12 (4) : 343 TFEB regulates pluripotency transcriptional network in mouse embryonic stem cells independent of autophagy-lysosomal biogenesis.
Application : WB, IP
Reactivity : Human
Heib T et al. Cell Rep 2021; 35 (6) : 109102 RhoA/Cdc42 signaling drives cytoplasmic maturation but not endomitosis in megakaryocytes.
Application : WB, IHC-Fr
Reactivity : Mouse
Cullmann K et al. Res Pract Thromb Haemost 2021; 5 (1) : 111-124 Forming megakaryocytes from murine-induced pluripotent stem cells by the inducible overexpression of supporting factors.
Moon S et al. EMBO Rep 2017; 18 (1) : 61-71 Phosphorylation by NLK inhibits YAP-14-3-3-interactions and induces its nuclear localization.
Application : WB
Reactivity : Human
Sprowl-Tanio S et al. Cancer Metab 2016; 4 : 20 Lactate/pyruvate transporter MCT-1 is a direct Wnt target that confers sensitivity to 3-bromopyruvate in colon cancer.
Application : WB
Reactivity : Human
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REVIEW

beta Tubulin antibody Cat. No. GTX107175

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cancer Metab in 2016.PMID: 27729975
Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Open Biol in 2014. PMID: 25392452
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169