beta Tubulin antibody

Name: beta Tubulin antibody
Brand: GeneTex
SKU: GTX107175
Price: 379.00 USD
Rating: 5 (2 reviews)

Anti-beta Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107175

Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175

Cat No. GTX107175

Reference 7

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-Fr, IP
Reactivity	Human, Mouse
Package	100 μl ($379), 25 μl ($169)

See all beta Tubulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-Fr	Assay dependent
IP	Assay dependent

Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3

Predict Reactivity

Bovine, Cat, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta Tubulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952436

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tubulin beta 1 class VI

Background

Microtubules are involved in a wide variety of cellular processes, including mitosis, morphogenesis, platelet formation, and mobility of cilia and flagella. Circulating platelets carry a single marginal microtubule coil that is wound in 8 to 12 turns and is responsible for platelet shape. TUBB1 is the major beta-tubulin expressed in platelets and megakaryocytes and is required for optimal platelet assembly (Wang et al., 1986 [PubMed 3782288]; Schulze et al., 2004 [PubMed 15315966]).[supplied by OMIM]

Database

Gene ID: 81027 TUBB1
UniProt: Q9H4B7 TUBB1

Research Area

DATA IMAGES

GTX107175 ICC/IF Image

beta Tubulin antibody detects beta Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Tubulin protein stained by beta Tubulin antibody (GTX107175) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX107175 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX107175 diluted at 1:1000

GTX107175 WB Image

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
B:Hep G2 (GTX27900)
10% SDS PAGE
GTX107175 diluted at 1:1000

REFERENCE

Application Reference

Anderson Tan et al. Cell Death Dis 2021; 12 (4) : 343 TFEB regulates pluripotency transcriptional network in mouse embryonic stem cells independent of autophagy-lysosomal biogenesis.

Application : WB, IP

Reactivity : Human

Heib T et al. Cell Rep 2021; 35 (6) : 109102 RhoA/Cdc42 signaling drives cytoplasmic maturation but not endomitosis in megakaryocytes.

Application : WB, IHC-Fr

Reactivity : Mouse

Cullmann K et al. Res Pract Thromb Haemost 2021; 5 (1) : 111-124 Forming megakaryocytes from murine-induced pluripotent stem cells by the inducible overexpression of supporting factors.

Hsin-Hsiung Chen et al. Oncotarget 2017; 8 (13) : 21501-21515 NRIP/DCAF6 stabilizes the androgen receptor protein by displacing DDB2 from the CUL4A-DDB1 E3 ligase complex in prostate cancer.

Application : WB

Reactivity : Human

Moon S et al. EMBO Rep 2017; 18 (1) : 61-71 Phosphorylation by NLK inhibits YAP-14-3-3-interactions and induces its nuclear localization.

Application : WB

Reactivity : Human

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