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beta Tubulin antibody

Anti-beta Tubulin antibody used in Western Blot (WB). GTX112658
Anti-beta Tubulin antibody used in Western Blot (WB). GTX112658
Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112658
Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112658
Anti-beta Tubulin antibody used in Western Blot (WB). GTX112658

Cat No. GTX112658

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , HepG2 , NIH-3T3 , JC , C2C12

Predict Reactivity

Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta Tubulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10730758

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

M40 antibody , MGC117247 antibody , MGC16435 antibody , OK/SW-cl.56 antibody , TUBB1 antibody , TUBB5 antibody , TUBB antibody , beta1-tubulin antibody , beta-4 tubulin antibody , tubulin beta-1 chain antibody , tubulin beta chain antibody , tubulin beta polypeptide antibody , "tubulin , beta polypeptide antibody" , class I beta-tubulin antibody , beta Ib tubulin antibody , beta 5-tubulin antibody , tubulin beta-5 chain antibody , "tubulin , beta antibody"

Research Area

DATA IMAGES

Anti-beta Tubulin antibody used in Western Blot (WB). GTX112658

GTX112658 WB Image

Sample (30 ug of whole cell lysate) 
      A: NIH-3T3
      B: JC
      C: C2C12
      10% SDS PAGE 
      GTX112658 diluted at 1:1000

Anti-beta Tubulin antibody used in Western Blot (WB). GTX112658

GTX112658 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2
10% SDS PAGE
GTX112658 diluted at 1:1000

Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112658

GTX112658 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using beta Tubulin(GTX112658) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112658

GTX112658 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using beta Tubulin(GTX112658) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-beta Tubulin antibody used in Western Blot (WB). GTX112658

GTX112658 WB Image

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
10% SDS PAGE
GTX112658 diluted at 1:10000

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf