APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:1000-1:10000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , HepG2 , NIH-3T3 , JC , C2C12
Predict Reactivity
Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human beta Tubulin. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10730758
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
M40 antibody , MGC117247 antibody , MGC16435 antibody , OK/SW-cl.56 antibody , TUBB1 antibody , TUBB5 antibody , TUBB antibody , beta1-tubulin antibody , beta-4 tubulin antibody , tubulin beta-1 chain antibody , tubulin beta chain antibody , tubulin beta polypeptide antibody , "tubulin , beta polypeptide antibody" , class I beta-tubulin antibody , beta Ib tubulin antibody , beta 5-tubulin antibody , tubulin beta-5 chain antibody , "tubulin , beta antibody"
Research Area
DATA IMAGES
|
GTX112658 WB Image
Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: C2C12
10% SDS PAGE
GTX112658 diluted at 1:1000
|
|
GTX112658 WB Image
Sample (30 ug of whole cell lysate)
A: Hep G2
10% SDS PAGE
GTX112658 diluted at 1:1000
|
|
GTX112658 IHC-P Image
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using beta Tubulin(GTX112658) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
GTX112658 IHC-P Image
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using beta Tubulin(GTX112658) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
GTX112658 WB Image
Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
10% SDS PAGE
GTX112658 diluted at 1:10000
|
REFERENCE
REVIEW
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![Anti-alpha Tubulin antibody [GT114] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628802](/upload/website/prouct_img/small/GTX628802/GTX628802_42030_20161004_IFA_18121411_142.jpg "Anti-alpha Tubulin antibody [GT114] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628802")
