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c-Abl (phospho Tyr412) antibody

Anti-c-Abl (phospho Tyr412) antibody used in Western Blot (WB). GTX50859
Anti-c-Abl (phospho Tyr412) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50859
Anti-c-Abl (phospho Tyr412) antibody used in IHC (Paraffin sections) (IHC-P). GTX50859

Cat No. GTX50859

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

123 kDa. ( Note )

Specificity/Sensitivity

This antibody is raised against human c-Abl (isoform b) phosphorylated at Tyr412. Based on sequence homology, it is predicted to react with c-Abl (isoform a) or ABL2 when phosphorylated at the corresponding residues - c-Abl (isoform a) Tyr393 or ABL2 Tyr439.

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 412 (D-T-Y(p)-T-A) derived from Human c-Abl.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ABL proto-oncogene 1, non-receptor tyrosine kinase , ABL , CHDSKM , JTK7 , bcr/abl , c-ABL , c-ABL1 , p150 , v-abl

Cellular Localization

Cytoplasm, cytoskeleton,Nucleus,Mitochondrion

Background

This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons. [provided by RefSeq, Aug 2014]

Database

Research Area

DATA IMAGES

Anti-c-Abl (phospho Tyr412) antibody used in Western Blot (WB). GTX50859

GTX50859 WB Image

WB analysis of extracts from HL-60 cells using GTX50859 c-Abl (phospho Tyr412) antibody with or without blocking peptide.

Anti-c-Abl (phospho Tyr412) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50859

GTX50859 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50859 c-Abl (phospho Tyr412) antibody.

Anti-c-Abl (phospho Tyr412) antibody used in IHC (Paraffin sections) (IHC-P). GTX50859

GTX50859 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50859 c-Abl (phospho Tyr412) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

There are currently no references for c-Abl (phospho Tyr412) antibody (GTX50859). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for c-Abl (phospho Tyr412) antibody (GTX50859). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf