APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:1000 |
| 1:100-1:200 |
| 1:50-1:100 |
Not tested in other applications.
Calculated MW
Product Note
This antibody is raised against human c-Abl (isoform b) phosphorylated at Tyr412. Based on sequence homology, it is predicted to react with c-Abl (isoform a) or ABL2 when phosphorylated at the corresponding residues - c-Abl (isoform a) Tyr393 or ABL2 Tyr439.
Predict Reactivity
Mouse(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide sequence around phosphorylation site of tyrosine 412 (D-T-Y(p)-T-A) derived from Human c-Abl.
Purification
Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ABL proto-oncogene 1, non-receptor tyrosine kinase , ABL , CHDSKM , JTK7 , bcr/abl , c-ABL , c-ABL1 , p150 , v-abl
Cellular Localization
Cytoplasm, cytoskeleton,Nucleus,Mitochondrion
Background
This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons. [provided by RefSeq, Aug 2014]
Database
Research Area
DATA IMAGES
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GTX50859 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX50859 c-Abl (phospho Tyr412) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide
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GTX50859 WB Image
WB analysis of extracts from HL-60 cells using GTX50859 c-Abl (phospho Tyr412) antibody with or without blocking peptide.
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GTX50859 ICC/IF Image
ICC/IF analysis of methanol-fixed HeLa cells using GTX50859 c-Abl (phospho Tyr412) antibody.
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REFERENCE
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REVIEW
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