APPLICATION
Application Note
Not suitable for IHC frozen or parraffin-embedded sections. For ICC: Use at a concentration of 2.5 - 5.0 μg/ml. For WB: Use at a concentration of 2.5 - 5.0 μg/ml. Detects bands of approximately 55 and 62 kDa. Optimal dilutions/concentrations should be determined by the researcher.
Calculated MW
Positive Control
HeLa cells
Product Note
Reacts with both cellular and viral forms, will detect both the 55 kDa and 62 kDa c-fos and v-fos proteins, but does not react with the 39 kDa jun protein.
PROPERTIES
Form
Liquid
Buffer
50mM Sodium Phosphate, 0.2% Gelatin
Preservative
0.1% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide: KVEQLSPEEEEKRRIRRERNKMAAA, corresponding to Internal sequence amino acids 128 - 152 of Human c-Fos.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Fos proto-oncogene, AP-1 transcription factor subunit , AP-1 , C-FOS , p55
Cellular Localization
Nucleus , Endoplasmic reticulum , Cytoplasm, cytosol
Background
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008]
Database
Research Area
REFERENCE
REVIEW
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