GeneTex
United States (US)

c-Fos antibody [2G9C3]

Cat No. GTX16902

Host Mouse
Clonality Monoclonal
Clone Name 2G9C3
Isotype IgG2a
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

Not suitable for IHC frozen or parraffin-embedded sections. For ICC: Use at a concentration of 2.5 - 5.0 μg/ml. For WB: Use at a concentration of 2.5 - 5.0 μg/ml. Detects bands of approximately 55 and 62 kDa. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

55 - 62 kD kDa. ( Note )

Positive Control

HeLa cells

Specificity/Sensitivity

Reacts with both cellular and viral forms, will detect both the 55 kDa and 62 kDa c-fos and v-fos proteins, but does not react with the 39 kDa jun protein.
PROPERTIES

Form

Liquid

Buffer

50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5 with ²0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: KVEQLSPEEEEKRRIRRERNKMAAA, corresponding to Internal sequence amino acids 128 - 152 of Human c-Fos.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Fos Proto-Oncogene, Ap-1 Transcription Factor Subunit,Ap-1,C-Fos,P55,Fos

Cellular Localization

Nuclear

Background

The Fos gene family consists of 4 members: Fos, FosB, FosL1, and FosL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP1. As such, the Fos proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the Fos gene has also been associated with apoptotic cell death. The human oncogene c-Fos is homologous to the Finkel-Biskis-Jinkins (FBJ) murine osteosarcoma virus oncogene. Fos was the first transcription factor identified that has a critical function in regulating the development of cells destined to form and maintain the skeleton.

Database

Research Area

Package List Price ($)
$ 319