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c-Fos antibody [2H2]

Anti-c-Fos antibody [2H2] used in IHC (Paraffin sections) (IHC-P). GTX60996
Anti-c-Fos antibody [2H2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60996
Anti-c-Fos antibody [2H2] used in Western Blot (WB). GTX60996
Anti-c-Fos antibody [2H2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60996
Anti-c-Fos antibody [2H2] used in IHC (Paraffin sections) (IHC-P). GTX60996
Anti-c-Fos antibody [2H2] used in IHC (Frozen sections) (IHC-Fr). GTX60996
Anti-c-Fos antibody [2H2] used in IHC (Paraffin sections) (IHC-P). GTX60996

Cat. No. GTX60996

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2H2

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all c-Fos products

APPLICATION

Application Note

Western blots: 1:1,000-2,000, ICC/IF or IHC-Fr: 1:1,000

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length recombinant human protein expressed in and purified from E. coli.

Purification

Affinity purification

Conjugation

Unconjugated

RRID

AB_2810303

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Fos proto-oncogene, AP-1 transcription factor subunit , AP-1 , C-FOS , p55

Cellular Localization

Nucleus , Endoplasmic reticulum , Cytoplasm, cytosol

Background

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-c-Fos antibody [2H2] used in IHC (Paraffin sections) (IHC-P). GTX60996

GTX60996 IHC-P Image

c-Fos antibody [2H2] detects c-Fos protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
c-Fos stained by c-Fos antibody [2H2] (GTX60996) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-c-Fos antibody [2H2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60996

GTX60996 ICC/IF Image

ICC/IF analysis of rat brain neural cultured cells using GTX60996 c-Fos antibody [2H2].
Left : Untreated cells
Right : Cells stimulated with membrane deplorization buffer for 5 hours.
Green : Primary antibody
Red : GFAP antibody
Blue : DAPI

Anti-c-Fos antibody [2H2] used in Western Blot (WB). GTX60996

GTX60996 WB Image

WB analysis of various samples using GTX60996 c-Fos antibody [2H2].
Lane 1: Protein marker (red)
Lane 2: HeLa cells in serum free media
Lane 3: HeLa cells stimulated with 20% FBS for 2hrs after 36hrs in serum free media.
Lane 4: Rat cortical neurons
Lane 5: Rat cortical neurons treated with membrane depolarization buffer for 5hrs.
Dilution : 1:1000

Anti-c-Fos antibody [2H2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60996

GTX60996 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60996 c-Fos antibody [2H2].
Left : Serum-starved HeLa cells
Right : Serum-starved and then stimulated with 20% FBS for 2 hours
Green : Primary antibody
Red : Vimentin antibody
Blue : DAPI
Dilution : 1:1000

Anti-c-Fos antibody [2H2] used in IHC (Paraffin sections) (IHC-P). GTX60996

GTX60996 IHC-P Image

IHC-P analysis of rat hippocampus tissue using using GTX60996 c-Fos antibody [2H2]
Antigen retrieval : Citrate buffer
Dilution : 1:1000

Anti-c-Fos antibody [2H2] used in IHC (Frozen sections) (IHC-Fr). GTX60996

GTX60996 IHC-Fr Image

IHC-Fr analysis of rat hippocampus tissue using using GTX60996 c-Fos antibody [2H2]
Red : Primary antibody
Green : FOX3 antibod
Blue : DAPI
Dilution : 1:200

Anti-c-Fos antibody [2H2] used in IHC (Paraffin sections) (IHC-P). GTX60996

GTX60996 IHC-P Image

IHC-P analysis of mouse hippocampus tissue using using GTX60996 c-Fos antibody [2H2]
Antigen retrieval : Citrate buffer
Dilution : 1:1000

REFERENCE

Application Reference

Chang CH et al. Cell Rep 2019; 28 (5) : 1195-1205.e3 The Ventral Hippocampus Controls Stress-Provoked Impulsive Aggression through the Ventromedial Hypothalamus in Post-Weaning Social Isolation Mice.
Application : IHC-Fr
Reactivity : Mouse

Clone Reference

Zhou Y et al. bioRxiv 2023; Activity Hierarchy Measurement to Establish Trace Memory-eligible "Primed" Neurons
Application : IHC-Fr
Reactivity : Mouse
Sommer S et al. Int J Neuropsychopharmacol 2022; 25 (3):173–184 Effects of Motivational Downshifts on Specific Pavlovian-Instrumental Transfer in Rats
Application : IHC-Fr
Reactivity : Rat
Keenan WT et al. Front Neural Circuits 2017; 11:93 Eye-Drops for Activation of DREADDs
Application : IHC-Fr
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations