c-Fos antibody [2H2]

c-Fos antibody [2H2] detects c-Fos protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
c-Fos stained by c-Fos antibody [2H2] (GTX60996) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

IHC-P analysis of mouse hippocampus tissue using using GTX60996 c-Fos antibody [2H2]
Antigen retrieval : Citrate buffer
Dilution : 1:1000

IHC-P analysis of rat hippocampus tissue using using GTX60996 c-Fos antibody [2H2]
Antigen retrieval : Citrate buffer
Dilution : 1:1000

ICC/IF analysis of HeLa cells using GTX60996 c-Fos antibody [2H2].
Left : Serum-starved HeLa cells
Right : Serum-starved and then stimulated with 20% FBS for 2 hours
Green : Primary antibody
Red : Vimentin antibody
Blue : DAPI
Dilution : 1:1000

WB analysis of various samples using GTX60996 c-Fos antibody [2H2].
Lane 1: Protein marker (red)
Lane 2: HeLa cells in serum free media
Lane 3: HeLa cells stimulated with 20% FBS for 2hrs after 36hrs in serum free media.
Lane 4: Rat cortical neurons
Lane 5: Rat cortical neurons treated with membrane depolarization buffer for 5hrs.
Dilution : 1:1000

ICC/IF analysis of rat brain neural cultured cells using GTX60996 c-Fos antibody [2H2].
Left : Untreated cells
Right : Cells stimulated with membrane deplorization buffer for 5 hours.
Green : Primary antibody
Red : GFAP antibody
Blue : DAPI