APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% glycerol
Preservative
5mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
Full length recombinant human protein expressed in and purified from E. coli.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Fos proto-oncogene, AP-1 transcription factor subunit , AP-1 , C-FOS , p55
Cellular Localization
Nucleus , Endoplasmic reticulum , Cytoplasm, cytosol
Background
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX03375 WB Image
WB analysis of various sample lysates using GTX03375 c-Fos antibody. Multiple bands at 50-65 kDa in stimulated or treated cell lysates, correspond to different isoforms of the c-FOS protein.
Red : Primary antibody
Green : GAPDH
Dilution : 1:2000
Lane 1 : protein marker
Lane 2 : HeLa cells grown in FBS free media
Lane 3 : HeLa cells stimulated with 20% FBS for 2 hours after being in FBS free media for 36 hours
Lane 4 : rat cortical neurons
Lane 5 : rat cortical neurons treated with membrane depolarization buffer for 5 hours
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GTX03375 IHC (Free Floating) Image
IHC (Free Floating) analysis of Mouse hippocampus (C) or olfactory bulb (D) tissue sections using GTX03375 c-Fos antibody. The c-FOS antibody stains only nuclei of spontaneously active neurons. NF-L is expressed in axons of neuronal cells.
Red : Primary antibody
Green : NF-L
Blue : Hoechst staining of nuclear DNA
Dilution : 1:10000
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GTX03375 ICC/IF Image
ICC/IF analysis of HeLa cells were treated with PBS (A) or stimulated with 20% FBS (B) for 30 min after serum stravation for 36hr using GTX03375 c-Fos antibody. c-FOS antibody labels only the nuclei of stimulated cells.
Red : Primary antibody
Green : Tubulin
Blue : DAPI
Dilution : 1:2000
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REFERENCE
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REVIEW
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