*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
MCF-7 treat 500nM PMA 0.5hr , MCF-7 treat 500nM PMA 1hr , MCF-7 treat 500nM PMA 2hr
Mouse, Sheep, Bovine, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human c-Fos. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Fos proto-oncogene, AP-1 transcription factor subunit,AP-1,C-FOS,p55
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq]
GTX101196 WB Image
c-Fos antibody detects FOS protein by Western blot analysis.
A. 30 µg MCF-7 whole cell lysate/extract (serum starvation)
B. 30 µg MCF-7 whole cell lysate/extract (500nM PMA treatment for 0.5hr)
C. 30 µg MCF-7 whole cell lysate/extract (500nM PMA treatment for 1hr)
D. 30 µg MCF-7 whole cell lysate/extract (500nM PMA treatment for 2hr)
10 % SDS-PAGE
c-Fos antibody (GTX101196) dilution: 1:5000
GTX101196 ICC/IF Image
c-Fos antibody detects c-Fos protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: c-Fos stained by c-Fos antibody (GTX101196) diluted at 1:1500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
GTX101196 ICC/IF Image
c-Fos antibody detects c-Fos protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: c-Fos protein stained by c-Fos antibody (GTX101196) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Scale bar = 10 μm.