GeneTex
United States (US)

c-Fos antibody

Orthogonal
GTX101196_40954_WB_19011506_148.jpg
GTX101196_40954_20161101_IFA_19011506_181.jpg
GTX101196_40954_20181115_ICC_IF_R_19011519_667.jpg

Cat No. GTX101196

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

MCF-7 treat 500nM PMA 0.5hr , MCF-7 treat 500nM PMA 1hr , MCF-7 treat 500nM PMA 2hr

Predict Reactivity

Mouse, Sheep, Bovine, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human c-Fos. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Fos Proto-Oncogene, Ap-1 Transcription Factor Subunit,Ap-1,C-Fos,P55,Fos

Cellular Localization

Nucleus

Background

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101196_40954_WB_19011506_148.jpg

GTX101196 WB Image

Orthogonal

c-Fos antibody detects FOS protein by Western blot analysis.
A. 30 µg MCF-7 whole cell lysate/extract (serum starvation)
B. 30 µg MCF-7 whole cell lysate/extract (500nM PMA treatment for 0.5hr)
C. 30 µg MCF-7 whole cell lysate/extract (500nM PMA treatment for 1hr)
D. 30 µg MCF-7 whole cell lysate/extract (500nM PMA treatment for 2hr)
10 % SDS-PAGE
c-Fos antibody (GTX101196) dilution: 1:5000

GTX101196_40954_20161101_IFA_19011506_181.jpg

GTX101196 ICC/IF Image

c-Fos antibody detects c-Fos protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: c-Fos protein stained by c-Fos antibody (GTX101196) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Scale bar = 10 μm.

GTX101196_40954_20181115_ICC_IF_R_19011519_667.jpg

GTX101196 ICC/IF Image

c-Fos antibody detects c-Fos protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: c-Fos stained by c-Fos antibody (GTX101196) diluted at 1:1500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169