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c-Fos antibody

Anti-c-Fos antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129846
Anti-c-Fos antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129846
Anti-c-Fos antibody used in Western Blot (WB). GTX129846
Anti-c-Fos antibody used in IHC (Frozen sections) (IHC-Fr). GTX129846

Cat No. GTX129846

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Predict Reactivity

Sheep, Bovine, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human c-Fos. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Fos proto-oncogene, AP-1 transcription factor subunit , AP-1 , C-FOS , p55

Cellular Localization

Nucleus

Background

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-c-Fos antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129846

GTX129846 ICC/IF Image

c-Fos antibody detects c-Fos protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: c-Fos stained by c-Fos antibody (GTX129846) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-c-Fos antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129846

GTX129846 ICC/IF Image

c-Fos antibody detects c-Fos protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: c-Fos protein stained by c-Fos antibody (GTX129846) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Scale bar = 10 μm.

Anti-c-Fos antibody used in Western Blot (WB). GTX129846

GTX129846 WB Image

Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with c-Fos antibody (GTX129846) diluted at 1:1000.

Anti-c-Fos antibody used in IHC (Frozen sections) (IHC-Fr). GTX129846

GTX129846 IHC-Fr Image

c-Fos antibody detects c-Fos protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebral cortex.
Green: c-Fos stained by c-Fos antibody (GTX129846) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169