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c-Fos antibody

Anti-c-Fos antibody used in Western Blot (WB). GTX25794
Anti-c-Fos antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25794
Anti-c-Fos antibody used in Western Blot (WB). GTX25794

Cat No. GTX25794

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse
Package
200 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
ICC/IF 1:500
IHC-P Assay dependent
FACS 3-5 μg/10⁶ cells
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues S(4) G F N A D Y E A S S S R C(17) of human cFos.

Purification

Ammonium sulfate precipitation

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

FBJ osteosarcoma oncogene , D12Rfj1 , c-fos , cFos

Cellular Localization

Nucleus,Endoplasmic reticulum,Cytoplasm, cytosol

Background

Cellular oncogenes, or proto-oncogenes, play pivotal roles in cellular communication pathways that regulate normal growth, development and differentiation. The cellular oncogene families fos and jun encode nuclear proteins that can function as transcription factors. The fos family of nuclear oncogenes encode cFos, FosB, (fos-related antigen) Fra1, and Fra2. Fos and Jun dimerize to form Activator Protein-1 (AP-1), a transcriptional factor that binds to the 12-O-tetradecanoylphorbol 13-acetate (TPA) response element (TRE) of several cellular and viral genes including human collagenase, metallothionein IIa, stromelysin, interleukin 2, SV40 and polyoma. Fos and Jun contain the ‘leucine-zipper’ motif that allows for dimerization and an adjacent basic domain required for biological activity. The functionally active form of Fos is in a heterodimer with a member of the Jun family. While Jun family members can form functional homodimers, studies indicate that Fos family members do not self-associate and therefore do not bind DNA on their own. The various dimers differ in their ability to transactivate AP-1 dependent genes.

Database

Research Area

DATA IMAGES

Anti-c-Fos antibody used in Western Blot (WB). GTX25794

GTX25794 WB Image

WB analysis of whole cell extracts (30 μg lysate) of HEL 92.1.7 (Lane 1), Jurkat (Lane 2), HeLa (Lane 3), HeLa treated for 20 minutes with 200 mM of TPA (Lane 4), K562 (Lane 5), KARPAS-299 (Lane 6) and HT-29 (Lane 7) using GTX25794 c-Fos antibody.
Dilution : 1-3 μg/ml

Anti-c-Fos antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25794

GTX25794 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX25794 c-Fos antibody.
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature
Dilution : 1:500 for at least 1 hour at room temperature

Anti-c-Fos antibody used in Western Blot (WB). GTX25794

GTX25794 WB Image

WB analysis of 50 ug of serum (20% Serum, 2 hours following 0.25% serum starvation, 36 hours) treated HeLa and 293T whole cell lysate using GTX25794 c-Fos antibody.
Dilution : 1:500

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339