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c-Fos (phospho Ser362) antibody

Anti-c-Fos (phospho Ser362) antibody used in IHC (Paraffin sections) (IHC-P). GTX32391
Anti-c-Fos (phospho Ser362) antibody used in Western Blot (WB). GTX32391

Cat. No. GTX32391

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all c-Fos products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of c-Fos. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Fos proto-oncogene, AP-1 transcription factor subunit , AP-1 , C-FOS , p55

Cellular Localization

Nucleus,Endoplasmic reticulum,Cytoplasm, cytosol

Background

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-c-Fos (phospho Ser362) antibody used in IHC (Paraffin sections) (IHC-P). GTX32391

GTX32391 IHC-P Image

IHC-P analysis of formalin fixed human lung cancer tissue section using GTX32391 c-Fos (phospho Ser362) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-c-Fos (phospho Ser362) antibody used in Western Blot (WB). GTX32391

GTX32391 WB Image

WB analysis of HepG2 (A), Adriamycin-treated HeLa (B) whole cell lysates using GTX32391 c-Fos (phospho Ser362) antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399