c-Fos (phospho Thr232) antibody

Cat. No. GTX17933

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, ChIP assay
Reactivity	Human
Package	50 μl ($399)

See all c-Fos products

Datasheet
Document

View product citations for antibody GTX17933 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	1:250
IHC-P	1:20
ChIP assay	1-3 μg/10⁶ cells

Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human c-Fos that contains threonine 232. The sequence is conserved in mouse and rat.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Fos proto-oncogene, AP-1 transcription factor subunit , AP-1 , C-FOS , p55

Cellular Localization

Nucleus,Endoplasmic reticulum,Cytoplasm, cytosol

Background

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008]

Database

Gene ID: 2353 FOS
UniProt: P01100 FOS

Research Area

DATA IMAGES

GTX17933 WB Image

WB (peptide competition) analysis of SKBR3 cells treated with 200ng/mL EGF for 10 minutes using GTX17933 c-Fos (phospho Thr232) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 2), a generic phosphothreonine-containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

GTX17933 ChIP assay Image

ChIP analysis of 2 million A431 cells treated with EGF (200ng/ml) for 30 minutes using GTX17933 c-Fos (phospho Thr232) antibody. Normal Rabbit IgG was used as a negative IP control. The precipitated DNA was detected by PCR with primer set targeting to the promoter of IL-6, MMP9 gene, used as positive control target, and the SAT2 used as negative control target.

GTX17933 ICC/IF Image

ICC/IF analysis of HeLa cells treated with 20 ng of EGF for 10 minutes using GTX17933 c-Fos (phospho Thr232) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes

GTX17933 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX17933 c-Fos (phospho Thr232) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

REFERENCE

There are currently no references for c-Fos (phospho Thr232) antibody (GTX17933). Be the first to share your publications with this product.

Submit a Reference

REVIEW

There are currently no reviews for c-Fos (phospho Thr232) antibody (GTX17933). Be the first to share your experience with this product.

Submit a Review

SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

50 μl

$ 399

Qty