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c-Jun antibody [5B1]

Anti-c-Jun antibody [5B1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60477
Anti-c-Jun antibody [5B1] used in Western Blot (WB). GTX60477
Anti-c-Jun antibody [5B1] used in Western Blot (WB). GTX60477
Anti-c-Jun antibody [5B1] used in IHC (Paraffin sections) (IHC-P). GTX60477
Anti-c-Jun antibody [5B1] used in IHC (Paraffin sections) (IHC-P). GTX60477
Anti-c-Jun antibody [5B1] used in Flow cytometry (FACS). GTX60477
Anti-c-Jun antibody [5B1] used in ELISA (ELISA). GTX60477

Cat No. GTX60477

Host

Mouse

Clonality

Monoclonal

Clone Name

5B1

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

Human, Mouse, Monkey
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites, 0.03% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human c-Jun expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_2887973

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Jun proto-oncogene, AP-1 transcription factor subunit , AP-1 , AP1 , c-Jun , p39

Cellular Localization

Nucleus

Background

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-c-Jun antibody [5B1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60477

GTX60477 ICC/IF Image

ICC/IF analysis of PC-2 cells using GTX60477 c-Jun antibody [5B1].
Green : c-Jun
Red: Actin filaments

Anti-c-Jun antibody [5B1] used in Western Blot (WB). GTX60477

GTX60477 WB Image

WB analysis of NIH3T3 (1) and Cos7 (2) cell lysate using GTX60477 c-Jun antibody [5B1].

Anti-c-Jun antibody [5B1] used in Western Blot (WB). GTX60477

GTX60477 WB Image

WB analysis of human c-Jun (AA: 199-331) recombinant protein using GTX60477 c-Jun antibody [5B1].

Anti-c-Jun antibody [5B1] used in IHC (Paraffin sections) (IHC-P). GTX60477

GTX60477 IHC-P Image

IHC-P analysis of human rectum cancer tissue using GTX60477 c-Jun antibody [5B1].

Anti-c-Jun antibody [5B1] used in IHC (Paraffin sections) (IHC-P). GTX60477

GTX60477 IHC-P Image

IHC-P analysis of human intima canncer tissue using GTX60477 c-Jun antibody [5B1].

Anti-c-Jun antibody [5B1] used in Flow cytometry (FACS). GTX60477

GTX60477 FACS Image

FACS analysis of HepG2 cells using GTX60477 c-Jun antibody [5B1].
Blue : c-Jun
Red : negative control

Anti-c-Jun antibody [5B1] used in ELISA (ELISA). GTX60477

GTX60477 ELISA Image

ELISA analysis of antigen using GTX60477 c-Jun antibody [5B1].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf