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c-Jun antibody

Anti-c-Jun antibody used in Western Blot (WB). GTX112974
Anti-c-Jun antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112974
Anti-c-Jun antibody used in IHC (Paraffin sections) (IHC-P). GTX112974

Cat. No. GTX112974

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all c-Jun products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Rabbit, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human c-Jun. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885325

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Jun proto-oncogene, AP-1 transcription factor subunit , AP-1 , AP1 , c-Jun , p39

Cellular Localization

Nucleus

Background

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-c-Jun antibody used in Western Blot (WB). GTX112974

GTX112974 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Jun antibody (GTX112974) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-c-Jun antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112974

GTX112974 ICC/IF Image

c-Jun antibody detects c-Jun protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: c-Jun stained by c-Jun antibody (GTX112974) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10 μm.

Anti-c-Jun antibody used in IHC (Paraffin sections) (IHC-P). GTX112974

GTX112974 IHC-P Image

c-Jun antibody detects c-Jun protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
c-Jun stained by c-Jun antibody (GTX112974) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Lee KT et al. Aging (Albany NY) 2021; 13 (18) : 22490-22501 Nesfatin-1 facilitates IL-1β production in osteoarthritis synovial fibroblasts by suppressing miR-204-5p synthesis through the AP-1 and NF-κB pathways.
Application : WB
Reactivity : Human
Lee H.P. et al. ournal of Functional Foods 2021; 86 : 104712 Cordycerebroside A suppresses VCAM-dependent monocyte adhesion in osteoarthritis synovial fibroblasts by inhibiting MEK/ERK/AP-1 signaling.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169