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c-Jun (phospho Ser63) antibody [GT653]

Orthogonal
Anti-c-Jun (phospho Ser63) antibody [GT653] used in Western Blot (WB). GTX634322
Anti-c-Jun (phospho Ser63) antibody [GT653] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634322
Anti-c-Jun (phospho Ser63) antibody [GT653] used in IHC (Paraffin sections) (IHC-P). GTX634322

Cat. No. GTX634322

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT653

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all c-Jun products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T (25 μg/ml Anisomycin treatment for 30 min)

Predict Reactivity

Mouse, Zebrafish, Rabbit, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser63 of human c-Jun. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888436

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Jun proto-oncogene, AP-1 transcription factor subunit , AP-1 , AP1 , c-Jun , p39

Cellular Localization

Nucleus

Background

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-c-Jun (phospho Ser63) antibody [GT653] used in Western Blot (WB). GTX634322

GTX634322 WB Image

Orthogonal

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Jun (phospho Ser63) antibody [GT653] (GTX634322) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-c-Jun (phospho Ser63) antibody [GT653] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634322

GTX634322 ICC/IF Image

c-Jun (phospho Ser63) antibody [GT653] detects c-Jun (phospho Ser63) protein at nucleus by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: c-Jun (phospho Ser63) protein stained by c-Jun (phospho Ser63) antibody [GT653] (GTX634322) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Anti-c-Jun (phospho Ser63) antibody [GT653] used in IHC (Paraffin sections) (IHC-P). GTX634322

GTX634322 IHC-P Image

c-Jun (phospho Ser63) antibody [GT653] detects c-Jun (phospho Ser63) protein at cytoplasm and nucleus in rat lung by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
c-Jun (phospho Ser63) antibody [GT653] (GTX634322) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Liu JF et al. J Cell Physiol 2020; (Epub) PM2.5 facilitates IL-6 production in human osteoarthritis synovial fibroblasts via ASK1 activation.
Application : WB
Reactivity : Human
Rodr?guez-Jorge O et al. Sci Signal 2019; 12 (577) Cooperation between T cell receptor and Toll-like receptor 5 signaling for CD4+ T cell activation.
Application : FACS
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations