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c-Jun (phospho Ser63) antibody [GT653]

Anti-c-Jun (phospho Ser63) antibody [GT653] used in Western Blot (WB). GTX634322
Anti-c-Jun (phospho Ser63) antibody [GT653] used in IHC (Paraffin sections) (IHC-P). GTX634322
Anti-c-Jun (phospho Ser63) antibody [GT653] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634322

Cat No. GTX634322

Host Mouse
Clonality Monoclonal
Clone Name GT653
Isotype IgG2a
Application WB, ICC/IF, IHC-P, FACS
Reactivity Human, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T (25 μg/ml Anisomycin treatment for 30 min)

Predict Reactivity

Mouse, Rabbit, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around c-Jun (phospho Ser63). The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Jun Proto-Oncogene, Ap-1 Transcription Factor Subunit,Ap-1,Ap1,C-Jun,P39,Jun

Cellular Localization

Nucleus

Background

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-c-Jun (phospho Ser63) antibody [GT653] used in Western Blot (WB). GTX634322

GTX634322 WB Image

Untreated (–) and treated (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Jun (phospho Ser63) antibody [GT653] (GTX634322) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-c-Jun (phospho Ser63) antibody [GT653] used in IHC (Paraffin sections) (IHC-P). GTX634322

GTX634322 IHC-P Image

c-Jun (phospho Ser63) antibody [GT653] detects c-Jun (phospho Ser63) protein at cytoplasm and nucleus in rat lung by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
c-Jun (phospho Ser63) antibody [GT653] (GTX634322) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-c-Jun (phospho Ser63) antibody [GT653] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634322

GTX634322 ICC/IF Image

c-Jun (phospho Ser63) antibody [GT653] detects c-Jun (phospho Ser63) protein at nucleus by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: c-Jun (phospho Ser63) protein stained by c-Jun (phospho Ser63) antibody [GT653] (GTX634322) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.

REFERENCE
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SDS
PBS.pdf