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c-Jun (phospho Ser73) antibody

Anti-c-Jun (phospho Ser73) antibody used in IHC (Paraffin sections) (IHC-P). GTX133873
Anti-c-Jun (phospho Ser73) antibody used in IHC (Paraffin sections) (IHC-P). GTX133873
Anti-c-Jun (phospho Ser73) antibody used in IHC (Paraffin sections) (IHC-P). GTX133873
Anti-c-Jun (phospho Ser73) antibody used in Western Blot (WB). GTX133873
Anti-c-Jun (phospho Ser73) antibody used in Western Blot (WB). GTX133873
Anti-c-Jun (phospho Ser73) antibody used in Western Blot (WB). GTX133873
Anti-c-Jun (phospho Ser73) antibody used in Western Blot (WB). GTX133873
Anti-c-Jun (phospho Ser73) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133873

Cat No. GTX133873

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 , HeLa (25 μg/ml Anisomycin treatment for 30 min)

Predict Reactivity

Zebrafish, Rabbit, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human c-Jun (phospho Ser73). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Jun proto-oncogene, AP-1 transcription factor subunit , AP-1 , AP1 , c-Jun , p39

Cellular Localization

Nucleus

Background

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-c-Jun (phospho Ser73) antibody used in IHC (Paraffin sections) (IHC-P). GTX133873

GTX133873 IHC-P Image

c-Jun (phospho Ser73) antibody detects c-Jun (phospho Ser73) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
c-Jun (phospho Ser73) stained by c-Jun (phospho Ser73) antibody (GTX133873) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-c-Jun (phospho Ser73) antibody used in IHC (Paraffin sections) (IHC-P). GTX133873

GTX133873 IHC-P Image

c-Jun (phospho Ser73) antibody detects c-Jun (phospho Ser73) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
c-Jun (phospho Ser73) stained by c-Jun (phospho Ser73) antibody (GTX133873) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-c-Jun (phospho Ser73) antibody used in IHC (Paraffin sections) (IHC-P). GTX133873

GTX133873 IHC-P Image

c-Jun (phospho Ser73) antibody detects c-Jun (phospho Ser73) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
c-Jun (phospho Ser73) stained by c-Jun (phospho Ser73) antibody (GTX133873) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-c-Jun (phospho Ser73) antibody used in Western Blot (WB). GTX133873

GTX133873 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Jun (phospho Ser73) antibody (GTX133873) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-c-Jun (phospho Ser73) antibody used in Western Blot (WB). GTX133873

GTX133873 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with c-Jun (phospho Ser73) antibody (GTX133873) diluted at 1:500 and competitor's antibody (CST#3270) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-c-Jun (phospho Ser73) antibody used in Western Blot (WB). GTX133873

GTX133873 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Jun (phospho Ser73) antibody (GTX133873) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-c-Jun (phospho Ser73) antibody used in Western Blot (WB). GTX133873

GTX133873 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Jun (phospho Ser73) antibody (GTX133873) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-c-Jun (phospho Ser73) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133873

GTX133873 ICC/IF Image

c-Jun (phospho Ser73) antibody detects c-Jun (phospho Ser73) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: c-Jun (phospho Ser73) stained by c-Jun (phospho Ser73) antibody (GTX133873) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10μm.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf