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c-Jun (phospho Ser73) antibody

Anti-c-Jun (phospho Ser73) antibody used in IHC (Paraffin sections) (IHC-P). GTX50091
Anti-c-Jun (phospho Ser73) antibody used in Western Blot (WB). GTX50091
Anti-c-Jun (phospho Ser73) antibody used in Western Blot (WB). GTX50091

Cat. No. GTX50091

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399)
See all c-Jun products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 73 (L-A-S(p)-P-E) derived from human c-Jun.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

RRID

AB_11163582

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Jun proto-oncogene, AP-1 transcription factor subunit , AP-1 , AP1 , c-Jun , p39

Cellular Localization

Nucleus

Background

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-c-Jun (phospho Ser73) antibody used in IHC (Paraffin sections) (IHC-P). GTX50091

GTX50091 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50091 c-Jun (phospho Ser73) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-c-Jun (phospho Ser73) antibody used in Western Blot (WB). GTX50091

GTX50091 WB Image

WB analysis of extracts from PC14 cells untreated or treated with EGF using GTX50091 c-Jun (phospho Ser73) antibody.

Anti-c-Jun (phospho Ser73) antibody used in Western Blot (WB). GTX50091

GTX50091 WB Image

WB analysis of extracts from C6 cells treated with PMA or calf intestinal phosphatase (CIP) using GTX50091 c-Jun (phospho Ser73) antibody.

REFERENCE

Application Reference

Nishinaka T et al. Chem Biol Interact 2019; 302 : 36-45 Cooperative regulation of mouse aldose reductase (AKR1B3) gene transcription by Nrf2, TonEBP, and c-jun.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399