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c-Jun (phospho Thr239) antibody

Anti-c-Jun (phospho Thr239) antibody used in IHC (Paraffin sections) (IHC-P). GTX50103
Anti-c-Jun (phospho Thr239) antibody used in Western Blot (WB). GTX50103
Anti-c-Jun (phospho Thr239) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50103

Cat. No. GTX50103

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all c-Jun products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 239 (G-E-T(p)-P-P) derived from human c-Jun.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

RRID

AB_11168028

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Jun proto-oncogene, AP-1 transcription factor subunit , AP-1 , AP1 , c-Jun , p39

Cellular Localization

Nucleus

Background

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-c-Jun (phospho Thr239) antibody used in IHC (Paraffin sections) (IHC-P). GTX50103

GTX50103 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50103 c-Jun (phospho Thr239) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-c-Jun (phospho Thr239) antibody used in Western Blot (WB). GTX50103

GTX50103 WB Image

WB analysis of extracts from HeLa cells untreated or treated with UV using GTX50103 c-Jun (phospho Thr239) antibody.

Anti-c-Jun (phospho Thr239) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50103

GTX50103 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50103 c-Jun (phospho Thr239) antibody.

REFERENCE

Application Reference

Strassheim D et al. Cells 2020; 9 (2) c-Jun, Foxo3a, and c-Myc Transcription Factors are Key Regulators of ATP-Mediated Angiogenic Responses in Pulmonary Artery Vasa Vasorum Endothelial Cells.
Application : WB
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399