APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide of human c-Kit (D816V Mutant)
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
KIT proto-oncogene, receptor tyrosine kinase , C-Kit , CD117 , MASTC , PBT , SCFR
Cellular Localization
Cell membrane; Single-pass type I membrane protein,Cytoplasm
Background
This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-c-Kit (D816V Mutant) antibody [HL2653] used in Western Blot (WB). GTX639112](/templates/default/js/lightbox/images/loading.gif "Anti-c-Kit (D816V Mutant) antibody [HL2653] used in Western Blot (WB). GTX639112")
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GTX639112 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with c-Kit (D816V Mutant) antibody [HL2653] (GTX639112) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX639112 ICC/IF Image
c-Kit (D816V Mutant) antibody [HL2653] detects c-Kit (D816V Mutant) protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% PFA.Green: c-Kit (D816V Mutant) stained by c-Kit (D816V Mutant) antibody [HL2653] (GTX639112) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
There are currently no references for c-Kit (D816V Mutant) antibody [HL2653] (GTX639112). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for c-Kit (D816V Mutant) antibody [HL2653] (GTX639112). Be the first to share your experience with this product.
![Anti-c-Kit (D816V Mutant) antibody [HL2653] used in Western Blot (WB). GTX639112](/upload/website/prouct_img/small/GTX639112/GTX639112_45271_20240105_WB_B_24010821_755.webp "Anti-c-Kit (D816V Mutant) antibody [HL2653] used in Western Blot (WB). GTX639112")
![Anti-c-Kit (D816V Mutant) antibody [HL2653] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639112](/upload/website/prouct_img/small/GTX639112/GTX639112_T-45194_20231229_ICC_IF_B_24011618_957.webp "Anti-c-Kit (D816V Mutant) antibody [HL2653] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639112")
![Anti-c-Kit (D816V Mutant) antibody [HL2653] used in Western Blot (WB). GTX639112](/upload/website/prouct_img/normal/GTX639112/GTX639112_45271_20240105_WB_B_24010821_755.webp "Anti-c-Kit (D816V Mutant) antibody [HL2653] used in Western Blot (WB). GTX639112")
![Anti-c-Kit (D816V Mutant) antibody [HL2653] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639112](/upload/website/prouct_img/normal/GTX639112/GTX639112_T-45194_20231229_ICC_IF_B_24011618_957.webp "Anti-c-Kit (D816V Mutant) antibody [HL2653] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639112")