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c-Kit antibody [ACK2] (APC-Cy7)

Anti-c-Kit antibody [ACK2] (APC-Cy7) used in Flow cytometry (FACS). GTX01469-15

Cat. No. GTX01469-15

Host

Rat

Clonality

Monoclonal

Clone Name

ACK2

Isotype

IgG2b

Application

FACS

Reactivity

Mouse
Package
100 μg ($309)
PRODUCT

Summary

The ACK2 antibody is specific for CD117, also called c-Kit, a 145 kDa cytokine receptor important in the development of hematopoietic stem cells, in oogenesis, and for functional activity of immune cells such as NK and mast cells. c-Kit binds to a ligand known as stem cell factor (SCF), or alternatively as mast cell growth factor. Ligand binding promotes the activation (dimerization) and subsequent tyrosine kinase activity of the c-Kit receptor and triggers key survival, expansion and maturation signals during hematopoietic progenitor cell development. Conversely, shedding of extracellular domain of c-Kit receptor is reported to induce inactivation or apoptosis within these cells. The survival signaling activity of c-Kit confers a proto-oncogenic attribute to the receptor, as overexpression or mutations in this protein are associated with tumor development.The ACK2 antibody is widely utilized as a marker to identify hematopoietic progenitors, and to neutralize receptor-ligand binding in vitro and in vivo.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Assay dependent
Not tested in other applications.

Calculated MW

109 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄ (pH7.2), 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Allophycocyanin-Cyanine7 (APC-Cy7)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Cellular Localization

Cell membrane,Cytoplasm

Background

The c-Kit proto-oncogene is the cellular homolog of the transforming gene of a feline retrovirus (v-Kit). The c-kit protein includes characteristics of a protein kinase transmembrane receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-c-Kit antibody [ACK2] (APC-Cy7) used in Flow cytometry (FACS). GTX01469-15

GTX01469-15 FACS Image

FACS analysis of mouse C57Bl/6 bone marrow cells using GTX01469-15 c-Kit antibody [ACK2] (APC-Cy7).
Right panel : co-stained with c-Kit antibody [ACK2] (APC-Cy7) and Mouse CD45R antibody (violetFluor™ 450)
Left panel : co-stained with isotype control and Mouse CD45R antibody (violetFluor™ 450)
antibody amount : 0.25 μg (5 μl)

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 309