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c-Kit antibody [ACK2]

Cat. No. GTX01469

Reference 7

Host

 Rat

Clonality

 Monoclonal

Clone Name

 ACK2

Isotype

 IgG2b

Application

 ICC/IF, IHC-P, FACS, Depletion

Reactivity

 Human, Mouse, Rat
Package
100 μg ($149)
See all c-Kit products
PRODUCT

Summary

The ACK2 antibody is specific for CD117, also called c-Kit, a 145 kDa cytokine receptor important in the development of hematopoietic stem cells, in oogenesis, and for functional activity of immune cells such as NK and mast cells. c-Kit binds to a ligand known as stem cell factor (SCF), or alternatively as mast cell growth factor. Ligand binding promotes the activation (dimerization) and subsequent tyrosine kinase activity of the c-Kit receptor and triggers key survival, expansion and maturation signals during hematopoietic progenitor cell development. Conversely, shedding of extracellular domain of c-Kit receptor is reported to induce inactivation or apoptosis within these cells. The survival signaling activity of c-Kit confers a proto-oncogenic attribute to the receptor, as overexpression or mutations in this protein are associated with tumor development.The ACK2 antibody is widely utilized as a marker to identify hematopoietic progenitors, and to neutralize receptor-ligand binding in vitro and in vivo.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-P Assay dependent
FACS Assay dependent
Depletion Assay dependent
Inhibition assay Assay dependent
Not tested in other applications.

Calculated MW

109 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Purity

> 90% (determined by SDS-PAGE)

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Cell membrane,Cytoplasm

Background

The c-Kit proto-oncogene is the cellular homolog of the transforming gene of a feline retrovirus (v-Kit). The c-kit protein includes characteristics of a protein kinase transmembrane receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

REFERENCE

Clone Reference

2012; Serotonin 5-HT2B receptors are required for bone-marrow contribution to pulmonary arterial hypertension.
Application : FACS
Reactivity : Mouse
2012; Leukocyte-associated Ig-like receptor-1-deficient mice have an altered immune cell phenotype.
Application : FACS
Reactivity : Mouse
2011; Targeting the CXCR4-CXCL12 axis mobilizes autologous hematopoietic stem cells and prolongs islet allograft survival via programmed death ligand 1.
Reactivity : Mouse
2011; Neutrophil survival and c-kit(+)-progenitor proliferation in Staphylococcus aureus-infected skin wounds promote resolution.
Application : Depletion
Reactivity : Mouse
2011; Inhibition of ovarian KIT phosphorylation by the ovotoxicant 4-vinylcyclohexene diepoxide in rats.
Application :
Reactivity : Rat
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 149