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c-Kit antibody [KIT/982]

Anti-c-Kit antibody [KIT/982] used in Western Blot (WB). GTX34607
Anti-c-Kit antibody [KIT/982] used in Protein Array (Protein Array). GTX34607

Cat No. GTX34607

Host

Mouse

Clonality

Monoclonal

Clone Name

KIT/982

Isotype

IgG1

Application

WB, Protein Array

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/ml for 2 hours at RT
Protein Array Assay dependent
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment (around aa100-300) of human CD117 protein (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD117 , CKit , KIT , KIT protooncogene receptor tyrosine kinase , PBT , SCFR , c-Kit , KIT proto-oncogene, receptor tyrosine kinase

Cellular Localization

Cell membrane,Cytoplasm

Background

This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-c-Kit antibody [KIT/982] used in Western Blot (WB). GTX34607

GTX34607 WB Image

WB analysis of recombinant protein fragment of CD117 using GTX34607 c-Kit antibody [KIT/982].

Anti-c-Kit antibody [KIT/982] used in Protein Array (Protein Array). GTX34607

GTX34607 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using CD117 Mouse Monoclonal Antibody (KIT/982). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf