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c-Kit antibody, N-term

Anti-c-Kit antibody, N-term used in Western Blot (WB). GTX81335
Anti-c-Kit antibody, N-term used in Flow cytometry (FACS). GTX81335
Anti-c-Kit antibody, N-term used in Western Blot (WB). GTX81335
Anti-c-Kit antibody, N-term used in Flow cytometry (FACS). GTX81335
Anti-c-Kit antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX81335

Cat. No. GTX81335

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human, Mouse
Package
400 μl ($399)
See all c-Kit products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:10-1:50
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 106-135 amino acids from the N-terminal region of human KIT.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

KIT proto-oncogene, receptor tyrosine kinase , C-Kit , CD117 , MASTC , PBT , SCFR

Background

This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-c-Kit antibody, N-term used in Western Blot (WB). GTX81335

GTX81335 WB Image

WB analysis of HeLa whole cell lysate using GTX81335 c-Kit antibody, N-term.
Loading : 20 μg per lane
Dilution : 1:1000

Anti-c-Kit antibody, N-term used in Flow cytometry (FACS). GTX81335

GTX81335 FACS Image

FACS analysis of NCI-H460 cells using GTX81335 c-Kit antibody, N-term.
Green : primary antibody
Blue : negative control

Anti-c-Kit antibody, N-term used in Western Blot (WB). GTX81335

GTX81335 WB Image

WB analysis of mouse testis tissue lysate (35ug/lane) using GTX81335 c-Kit antibody, N-term.

Anti-c-Kit antibody, N-term used in Flow cytometry (FACS). GTX81335

GTX81335 FACS Image

FACS analysis of NCI-H460 cells using GTX81335 c-Kit antibody, N-term.
Top histogram : negative control
Bottom histogram : NCI-H460 cells

Anti-c-Kit antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX81335

GTX81335 IHC-P Image

IHC-P analysis of human skin tissue using GTX81335 c-Kit antibody, N-term.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399