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c-Kit antibody

Anti-c-Kit antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33947
Anti-c-Kit antibody used in Western Blot (WB). GTX33947
Anti-c-Kit antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33947
Anti-c-Kit antibody used in IHC (Paraffin sections) (IHC-P). GTX33947

Cat. No. GTX33947

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Pig
Package
100 μl ($399)
See all c-Kit products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:300-1000
ICC/IF 1:50-200
IHC-P 1:50-400
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide derived from human c-Kit(360-405).

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

KIT proto-oncogene, receptor tyrosine kinase , C-Kit , CD117 , MASTC , PBT , SCFR

Background

This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-c-Kit antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33947

GTX33947 ICC/IF Image

ICC/IF analysis of U138 cells using GTX33947 c-Kit antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 1:200

Anti-c-Kit antibody used in Western Blot (WB). GTX33947

GTX33947 WB Image

WB analysis of mouse pancreatic cancer tissue lysate using GTX33947 c-Kit antibody.
Dilution : 1:1000

Anti-c-Kit antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33947

GTX33947 ICC/IF Image

ICC/IF analysis of A431 cells using GTX33947 c-Kit antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 1:50

Anti-c-Kit antibody used in IHC (Paraffin sections) (IHC-P). GTX33947

GTX33947 IHC-P Image

IHC-P analysis of rat intestine tissue using GTX33947 c-Kit antibody.
Dilution : 1:200

REFERENCE

Application Reference

Bakheet N et al. Obes Surg 2020; 30 (11) : 4643-4651 A Novel Intragastric Satiety-Inducing Device to Inhibit Weight Gain in Juvenile Pigs: a Pilot Study.
Application : IHC-P
Reactivity : Pig
Submit a Reference

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399