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c-Kit (phospho Tyr730) antibody

Anti-c-Kit (phospho Tyr730) antibody used in Western Blot (WB). GTX25633
Anti-c-Kit (phospho Tyr730) antibody used in IHC (Paraffin sections) (IHC-P). GTX25633
Anti-c-Kit (phospho Tyr730) antibody used in Western Blot (WB). GTX25633

Cat No. GTX25633

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat, Bovine, Dog, Chicken
Package
50 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
IHC-P 1:10-1:50
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1mg/ml BSA, 50% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human c-Kit that contains tyrosine 730. The sequence is conserved in mouse, rat and cow.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

KIT proto-oncogene, receptor tyrosine kinase , C-Kit , CD117 , MASTC , PBT , SCFR

Background

This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-c-Kit (phospho Tyr730) antibody used in Western Blot (WB). GTX25633

GTX25633 WB Image

WB analysis of M07e cells left untreated (Lane 1) or treated (Lane 2-5) with SCF using GTX25633 c-Kit (phospho Tyr730) antibody.

Anti-c-Kit (phospho Tyr730) antibody used in IHC (Paraffin sections) (IHC-P). GTX25633

GTX25633 IHC-P Image

IHC-P analysis of human gastric carcinoma tissue using GTX25633 c-Kit (phospho Tyr730) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-c-Kit (phospho Tyr730) antibody used in Western Blot (WB). GTX25633

GTX25633 WB Image

WB analysis of 30 μg of A431 (Lane 1) treated for 10 minutes with 200 ng/ml of EGF (Lane 2), Rat Lung (Lane 3), HeLa (Lane 4), A549 (Lane 5) and serum starved A549 (Lane 6) lysate using GTX25633 c-Kit (phospho Tyr730) antibody.
Dilution : 1:1000

REFERENCE

There are currently no references for c-Kit (phospho Tyr730) antibody (GTX25633). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for c-Kit (phospho Tyr730) antibody (GTX25633). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf