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c-Kit (phospho Tyr936) antibody

Orthogonal
Anti-c-Kit (phospho Tyr936) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25635
Anti-c-Kit (phospho Tyr936) antibody used in Western Blot (WB). GTX25635
Anti-c-Kit (phospho Tyr936) antibody used in Western Blot (WB). GTX25635

Cat. No. GTX25635

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
50 μl ($399)
See all c-Kit products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:250
ICC/IF 1:250
FACS 1:20
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human c-Kit that contains tyrosine 936. The sequence is conserved in cow.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

KIT proto-oncogene, receptor tyrosine kinase , C-Kit , CD117 , MASTC , PBT , SCFR

Background

This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-c-Kit (phospho Tyr936) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25635

GTX25635 ICC/IF Image

ICC/IF analysis of A549 cells using GTX25635 c-Kit (phospho Tyr936) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1:250 dilution in 1% BSA incubated for 3 hours at room temperature

Anti-c-Kit (phospho Tyr936) antibody used in Western Blot (WB). GTX25635

GTX25635 WB Image

Orthogonal

WB analysis of whole cell extracts (30 μg lysate) of K562 (Lane 1) treated for 10 minutes with 100 ng/ml of SCF (Lane 2), HT 29 (Lane 3) treated for 10 minutes with 100 ng/ml of SCF (Lane 4) using GTX25635 c-Kit (phospho Tyr936) antibody.
Dilution : 1:250

Anti-c-Kit (phospho Tyr936) antibody used in Western Blot (WB). GTX25635

GTX25635 WB Image

Orthogonal

WB analysis of M07e cells left untreated (Lane 1) or treated (Lane 2-5) with SCF using GTX25635 c-Kit (phospho Tyr936) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399