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c-Kit (phospho Tyr936) antibody

Anti-c-Kit (phospho Tyr936) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25635
Anti-c-Kit (phospho Tyr936) antibody used in Western Blot (WB). GTX25635
Anti-c-Kit (phospho Tyr936) antibody used in Western Blot (WB). GTX25635

Cat No. GTX25635

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, FACS

Reactivity

Human
Package
50 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:250
ICC/IF 1:250
FACS 1:20
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 50% glycerol, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human c-Kit that contains tyrosine 936. The sequence is conserved in cow.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

KIT proto-oncogene, receptor tyrosine kinase , C-Kit , CD117 , MASTC , PBT , SCFR

Background

This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-c-Kit (phospho Tyr936) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25635

GTX25635 ICC/IF Image

ICC/IF analysis of A549 cells using GTX25635 c-Kit (phospho Tyr936) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1:250 dilution in 1% BSA incubated for 3 hours at room temperature

Anti-c-Kit (phospho Tyr936) antibody used in Western Blot (WB). GTX25635

GTX25635 WB Image

WB analysis of M07e cells left untreated (Lane 1) or treated (Lane 2-5) with SCF using GTX25635 c-Kit (phospho Tyr936) antibody.

Anti-c-Kit (phospho Tyr936) antibody used in Western Blot (WB). GTX25635

GTX25635 WB Image

WB analysis of whole cell extracts (30 μg lysate) of K562 (Lane 1) treated for 10 minutes with 100 ng/ml of SCF (Lane 2), HT 29 (Lane 3) treated for 10 minutes with 100 ng/ml of SCF (Lane 4) using GTX25635 c-Kit (phospho Tyr936) antibody.
Dilution : 1:250

REFERENCE

There are currently no references for c-Kit (phospho Tyr936) antibody (GTX25635). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for c-Kit (phospho Tyr936) antibody (GTX25635). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf