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c-Met antibody [C3], C-term

KO/KD
Anti-c-Met antibody [C3], C-term used in Western Blot (WB). GTX100637
Anti-c-Met antibody [C3], C-term used in Western Blot (WB). GTX100637
Anti-c-Met antibody [C3], C-term used in Western Blot (WB). GTX100637
Anti-c-Met antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100637
Anti-c-Met antibody [C3], C-term used in Immunoprecipitation (IP). GTX100637
Anti-c-Met antibody [C3], C-term used in Western Blot (WB). GTX100637

Cat No. GTX100637

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($329),
25 μl ($169)
See all c-Met products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

156 kDa. ( Note )

Positive Control

HeLa , HeLaS3

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human c-Met. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MET proto-oncogene, receptor tyrosine kinase , AUTS9 , DFNB97 , HGFR , RCCP2 , c-Met

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-c-Met antibody [C3], C-term used in Western Blot (WB). GTX100637

GTX100637 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [C3], C-term (GTX100637) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-c-Met antibody [C3], C-term used in Western Blot (WB). GTX100637

GTX100637 WB Image

Sample(30 μg whole cell lysate)
A:HeLa S3(GTX14654)
5% SDS PAGE
GTX100637 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-c-Met antibody [C3], C-term used in Western Blot (WB). GTX100637

GTX100637 WB Image

KO/KD

Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [C3], C-term (GTX100637) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-c-Met antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100637

GTX100637 ICC/IF Image

c-Met antibody [c3], c-term detects c-Met protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: c-Met protein stained by c-Met antibody [c3], c-term (GTX100637) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-c-Met antibody [C3], C-term used in Immunoprecipitation (IP). GTX100637

GTX100637 IP Image

Immunoprecipitation of c-Met protein from HeLa whole cell extracts using 5 μg of c-Met antibody [C3], C-term (GTX100637).
Western blot analysis was performed using c-Met antibody [C3], C-term (GTX100637) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-c-Met antibody [C3], C-term used in Western Blot (WB). GTX100637

GTX100637 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [C3], C-term (GTX100637) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Chen ST et al. Oncogene 2018; (Epub) Silencing of MUC20 suppresses the malignant character of pancreatic ductal adenocarcinoma cells through inhibition of the HGF/MET pathway.
Application : WB
Reactivity : Human
Pei YF et al. Biochim Biophys Acta 2017; (Epub) TOP2A induces malignant character of pancreatic cancer through activating β-catenin signaling pathway.
Application : WB
Reactivity : Human
Wang C et al. Exp Dermatol 2014; 23 (11) : 843-9 Loss of cell invasiveness through PKC-mediated syndecan-1 downregulation in melanoma cells under anchorage independency.
Application : WB
Reactivity : Human
Wu YM et al. Cancer Res 2013; 73 (17) : 5580-90 C1GALT1 enhances proliferation of hepatocellular carcinoma cells via modulating MET glycosylation and dimerization.
Application : WB
Reactivity : Human
Lin WC et al. Cancer Res 2010; 70 (14) : 1402746 Translocation of Helicobacter pylori CagA into Human B lymphocytes, the origin of mucosa-associated lymphoid tissue lymphoma.
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169