c-Met antibody [C3], C-term

GTX100637_39988_20160901_WB_shRNA_watermark_19011506_868.jpg

GTX100637_39988_20170601_WB_KO_watermark_19011506_799.jpg

Cat No. GTX100637

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IP
Reactivity	Human

See all c-Met products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

156 kDa. ( Note )

Positive Control

HeLa , HeLaS3

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human c-Met. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Met Proto-Oncogene, Receptor Tyrosine Kinase,Auts9,Dfnb97,Hgfr,Rccp2,C-Met,Met

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Gene ID: 4233
UniProt: P08581

Research Area

DATA IMAGES

GTX100637 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [C3], C-term (GTX100637) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100637 WB Image

Sample(30 µg whole cell lysate)
A:HeLa S3(GTX14654)
5% SDS PAGE
GTX100637 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100637 WB Image

Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [C3], C-term (GTX100637) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by

GTX100637 ICC/IF Image

c-Met antibody [c3], c-term detects c-Met protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: c-Met protein stained by c-Met antibody [c3], c-term (GTX100637) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX100637 IP Image

Immunoprecipitation of c-Met protein from HeLa whole cell extracts using 5 μg of c-Met antibody [C3], C-term (GTX100637).
Western blot analysis was performed using c-Met antibody [C3], C-term (GTX100637).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE