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c-Met antibody [GT1586]

Anti-c-Met antibody [GT1586] used in Western Blot (WB). GTX631993
Anti-c-Met antibody [GT1586] used in Western Blot (WB). GTX631993
Anti-c-Met antibody [GT1586] used in Immunoprecipitation (IP). GTX631993
Anti-c-Met antibody [GT1586] used in Western Blot (WB). GTX631993

Cat No. GTX631993

Host Mouse
Clonality Monoclonal
Clone Name GT1586
Isotype IgG1
Application WB, IP
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

156 kDa. ( Note )

Positive Control

HeLa , A431

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human c-Met. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MET proto-oncogene, receptor tyrosine kinase , AUTS9 , DFNB97 , HGFR , RCCP2 , c-Met

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-c-Met antibody [GT1586] used in Western Blot (WB). GTX631993

GTX631993 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT1586] (GTX631993) diluted at 1:500.

Anti-c-Met antibody [GT1586] used in Western Blot (WB). GTX631993

GTX631993 WB Image

Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT1586] (GTX631993) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-c-Met antibody [GT1586] used in Immunoprecipitation (IP). GTX631993

GTX631993 IP Image

Immunoprecipitation of c-Met protein from HeLa whole cell extracts using 5 μg of c-Met antibody [GT1586] (GTX631993).
Western blot analysis was performed using c-Met antibody [GT1586] (GTX631993).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-c-Met antibody [GT1586] used in Western Blot (WB). GTX631993

GTX631993 WB Image

c-Met antibody detects c-Met protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody (GTX631993) diluted by 1:1000.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169