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c-Met antibody [GT1586]

Anti-c-Met antibody [GT1586] used in Western Blot (WB). GTX631993
Anti-c-Met antibody [GT1586] used in Western Blot (WB). GTX631993
Anti-c-Met antibody [GT1586] used in Western Blot (WB). GTX631993
Anti-c-Met antibody [GT1586] used in Immunoprecipitation (IP). GTX631993

Cat. No. GTX631993

Host

Mouse

Clonality

Monoclonal

Clone Name

GT1586

Isotype

IgG1

Application

WB, IP

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

156 kDa. ( Note )

Positive Control

A431 , HeLa

Product Note

This antibody was raised against human c-Met Intracellular domain.

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human c-Met. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888271

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MET proto-oncogene, receptor tyrosine kinase , AUTS9 , DFNB97 , HGFR , RCCP2 , c-Met

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-c-Met antibody [GT1586] used in Western Blot (WB). GTX631993

GTX631993 WB Image

Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT1586] (GTX631993) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-c-Met antibody [GT1586] used in Western Blot (WB). GTX631993

GTX631993 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT1586] (GTX631993) diluted at 1:500.

Anti-c-Met antibody [GT1586] used in Western Blot (WB). GTX631993

GTX631993 WB Image

c-Met antibody detects c-Met protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody (GTX631993) diluted by 1:1000.

Anti-c-Met antibody [GT1586] used in Immunoprecipitation (IP). GTX631993

GTX631993 IP Image

Immunoprecipitation of c-Met protein from HeLa whole cell extracts using 5 μg of c-Met antibody [GT1586] (GTX631993).
Western blot analysis was performed using c-Met antibody [GT1586] (GTX631993).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

REFERENCE

There are currently no references for c-Met antibody [GT1586] (GTX631993). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for c-Met antibody [GT1586] (GTX631993). Be the first to share your experience with this product.
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations