APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
A431 , HeLa
Product Note
This antibody was raised against human c-Met Intracellular domain.
Predict Reactivity
Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide encompassing a sequence within the Intracellular domain of human c-Met. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888271
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
MET proto-oncogene, receptor tyrosine kinase , AUTS9 , DFNB97 , HGFR , RCCP2 , c-Met
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-c-Met antibody [GT1586] used in Western Blot (WB). GTX631993](/templates/default/js/lightbox/images/loading.gif "Anti-c-Met antibody [GT1586] used in Western Blot (WB). GTX631993")
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GTX631993 WB Image
Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT1586] (GTX631993) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX631993 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT1586] (GTX631993) diluted at 1:500.
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GTX631993 WB Image
c-Met antibody detects c-Met protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody (GTX631993) diluted by 1:1000.
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GTX631993 IP Image
Immunoprecipitation of c-Met protein from HeLa whole cell extracts using 5 μg of c-Met antibody [GT1586] (GTX631993).
Western blot analysis was performed using c-Met antibody [GT1586] (GTX631993).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
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REFERENCE
There are currently no references for c-Met antibody [GT1586] (GTX631993). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for c-Met antibody [GT1586] (GTX631993). Be the first to share your experience with this product.
![Anti-c-Met antibody [GT1586] used in Western Blot (WB). GTX631993](/upload/website/prouct_img/small/GTX631993/GTX631993_41904_20170601_WB_KO_watermark_w_23061202_426.webp "Anti-c-Met antibody [GT1586] used in Western Blot (WB). GTX631993")
![Anti-c-Met antibody [GT1586] used in Western Blot (WB). GTX631993](/upload/website/prouct_img/small/GTX631993/GTX631993_41904_20160901_WB_shRNA_watermark_w_23061202_294.webp "Anti-c-Met antibody [GT1586] used in Western Blot (WB). GTX631993")
![Anti-c-Met antibody [GT1586] used in Western Blot (WB). GTX631993](/upload/website/prouct_img/small/GTX631993/GTX631993_41904_20141121_WB_w_23061202_869.webp "Anti-c-Met antibody [GT1586] used in Western Blot (WB). GTX631993")
![Anti-c-Met antibody [GT1586] used in Immunoprecipitation (IP). GTX631993](/upload/website/prouct_img/small/GTX631993/GTX631993_41904_20150710_IP_w_23061202_300.webp "Anti-c-Met antibody [GT1586] used in Immunoprecipitation (IP). GTX631993")
![Anti-c-Met antibody [GT1586] used in Western Blot (WB). GTX631993](/upload/website/prouct_img/normal/GTX631993/GTX631993_41904_20170601_WB_KO_watermark_w_23061202_426.webp "Anti-c-Met antibody [GT1586] used in Western Blot (WB). GTX631993")
![Anti-c-Met antibody [GT1586] used in Western Blot (WB). GTX631993](/upload/website/prouct_img/normal/GTX631993/GTX631993_41904_20160901_WB_shRNA_watermark_w_23061202_294.webp "Anti-c-Met antibody [GT1586] used in Western Blot (WB). GTX631993")
![Anti-c-Met antibody [GT1586] used in Western Blot (WB). GTX631993](/upload/website/prouct_img/normal/GTX631993/GTX631993_41904_20141121_WB_w_23061202_869.webp "Anti-c-Met antibody [GT1586] used in Western Blot (WB). GTX631993")
![Anti-c-Met antibody [GT1586] used in Immunoprecipitation (IP). GTX631993](/upload/website/prouct_img/normal/GTX631993/GTX631993_41904_20150710_IP_w_23061202_300.webp "Anti-c-Met antibody [GT1586] used in Immunoprecipitation (IP). GTX631993")