c-Met antibody [GT556]

Name: c-Met antibody [GT556]
Brand: GeneTex
SKU: GTX631992
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-c-Met antibody [GT556] used in Western Blot (WB). GTX631992

Anti-c-Met antibody [GT556] used in IHC (Paraffin sections) (IHC-P). GTX631992

Anti-c-Met antibody [GT556] used in Immunoprecipitation (IP). GTX631992

Cat. No. GTX631992

Reference 1

Host	Mouse
Clonality	Monoclonal
Clone Name	GT556
Isotype	IgG2a
Application	WB, IHC-P, FACS, IP
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all c-Met products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000
FACS	Assay dependent
IP	1:100-1:500

Not tested in other applications.

Calculated MW

156 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human c-Met. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888270

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MET proto-oncogene, receptor tyrosine kinase , AUTS9 , DFNB97 , HGFR , RCCP2 , c-Met

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Gene ID: 4233 MET
UniProt: P08581 MET

Research Area

DATA IMAGES

GTX631992 WB Image

Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (GTX631992) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX631992 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (GTX631992) diluted at 1:500.

GTX631992 IHC-P Image

c-Met antibody [GT556] detects c-Met protein at cytoplasm in human esophageal carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
c-Met antibody [GT556] (GTX631992) diluted at 1:100.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX631992 IP Image

Immunoprecipitation of c-Met protein from HeLa whole cell extracts using 5 μg of MET antibody [GT556] (GTX631992).
Western blot analysis was performed using c-Met antibody [GT556] (GTX631992).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

GTX631992 IHC-P Image

c-Met antibody [GT556] detects c-Met protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
c-Met antibody [GT556] (GTX631992) diluted at 1:100.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX631992 WB Image

c-Met antibody detects c-Met protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody (GTX631992) diluted by 1:1000.

REFERENCE

Application Reference

Hung YH et al. Am J Cancer Res 2022; 12 (2) : 713-728 Semaphorin 4C promotes motility and immunosuppressive activity of cancer cells via CRMP3 and PD-L1.

Application : FACS

Reactivity : Human

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