Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human c-Met. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888270
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (GTX631992) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Show More +
GTX631992 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (GTX631992) diluted at 1:500.
Show More +
GTX631992 IHC-P Image
c-Met antibody [GT556] detects c-Met protein at cytoplasm in human esophageal carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human esophageal carcinoma. c-Met antibody [GT556] (GTX631992) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Show More +
GTX631992 IP Image
Immunoprecipitation of c-Met protein from HeLa whole cell extracts using 5 μg of MET antibody [GT556] (GTX631992). Western blot analysis was performed using c-Met antibody [GT556] (GTX631992). EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
Show More +
GTX631992 IHC-P Image
c-Met antibody [GT556] detects c-Met protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. c-Met antibody [GT556] (GTX631992) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Show More +
GTX631992 WB Image
c-Met antibody detects c-Met protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody (GTX631992) diluted by 1:1000.