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GeneTex
United States (US)

c-Met antibody [GT556]

KO/KD
GTX631992_41904_20170601_WB_KO_watermark_18121411_977.jpg
GTX631992_41904_20160901_WB_shRNA_watermark_18121411_844.jpg
GTX631992_41904_20151111_IHC-P_2_18121411_492.jpg
GTX631992_41904_20150710_IP_18121411_170.jpg
GTX631992_41904_20151111_IHC-P_18121411_705.jpg
GTX631992_41904_20141121_WB_18121411_336.jpg

Cat No. GTX631992

Host Mouse
Clonality Monoclonal
Clone Name GT556
Isotype IgG2a
Application WB, IHC-P, IP
Reactivity Human
See all c-Met products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

156 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human c-Met. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Met Proto-Oncogene, Receptor Tyrosine Kinase,Auts9,Dfnb97,Hgfr,Rccp2,C-Met,Met

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX631992_41904_20170601_WB_KO_watermark_18121411_977.jpg

GTX631992 WB Image

Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (GTX631992) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
CRISPR knockout cell lines generated by www.edigene.com

GTX631992_41904_20160901_WB_shRNA_watermark_18121411_844.jpg

GTX631992 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (GTX631992) diluted at 1:500.
GTX631992_41904_20151111_IHC-P_2_18121411_492.jpg

GTX631992 IHC-P Image

c-Met antibody [GT556] detects c-Met protein at cytoplasm in human esophageal carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
c-Met antibody [GT556] (GTX631992) diluted at 1:100.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX631992_41904_20150710_IP_18121411_170.jpg

GTX631992 IP Image

Immunoprecipitation of c-Met protein from HeLa whole cell extracts using 5 μg of MET antibody [GT556] (GTX631992).
Western blot analysis was performed using c-Met antibody [GT556] (GTX631992).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
GTX631992_41904_20151111_IHC-P_18121411_705.jpg

GTX631992 IHC-P Image

c-Met antibody [GT556] detects c-Met protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
c-Met antibody [GT556] (GTX631992) diluted at 1:100.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX631992_41904_20141121_WB_18121411_336.jpg

GTX631992 WB Image

c-Met antibody detects c-Met protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody (GTX631992) diluted by 1:1000.
SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169