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c-Met (phospho Tyr1234) antibody

Anti-c-Met (phospho Tyr1234) antibody used in Western Blot (WB). GTX02868
Anti-c-Met (phospho Tyr1234) antibody used in Western Blot (WB). GTX02868
Anti-c-Met (phospho Tyr1234) antibody used in ELISA (ELISA). GTX02868
Anti-c-Met (phospho Tyr1234) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02868

Cat No. GTX02868

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

156 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human c-Met around the phosphorylation site of Tyr1234.

Purification

Purified by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MET proto-oncogene, receptor tyrosine kinase , AUTS9 , DFNB97 , HGFR , RCCP2 , c-Met

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers. [provided by RefSeq, May 2016]

Database

Research Area

DATA IMAGES

Anti-c-Met (phospho Tyr1234) antibody used in Western Blot (WB). GTX02868

GTX02868 WB Image

WB analysis of extracts from heat-shock treated EC304 cells using GTX02868 c-Met (phospho Tyr1234) antibody. The lane on the left was treated with blocking peptide.

Anti-c-Met (phospho Tyr1234) antibody used in Western Blot (WB). GTX02868

GTX02868 WB Image

WB analysis of extracts from mouse lung and rat kidney using GTX02868 c-Met (phospho Tyr1234) antibody. -/+ means absence or presence of N peptide(non-phospho peptide) and P peptide(phospho peptide).

Anti-c-Met (phospho Tyr1234) antibody used in ELISA (ELISA). GTX02868

GTX02868 ELISA Image

ELISA analysis of phospho- and non phosho- immunogen peptide using GTX02868 c-Met (phospho Tyr1234) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml

Anti-c-Met (phospho Tyr1234) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02868

GTX02868 ICC/IF Image

ICC/IF analysis of PFA-fixed NIH-3T3 cells using GTX02868 c-Met (phospho Tyr1234) antibody.
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

REFERENCE

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REVIEW

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