APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH7.4, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human c-Met around the phosphorylation site of Tyr1234.
Purification
Purified by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns
From serum
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
MET proto-oncogene, receptor tyrosine kinase , AUTS9 , DFNB97 , HGFR , RCCP2 , c-Met
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers. [provided by RefSeq, May 2016]
Database
Research Area
DATA IMAGES
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GTX02868 WB Image
WB analysis of extracts from heat-shock treated EC304 cells using GTX02868 c-Met (phospho Tyr1234) antibody. The lane on the left was treated with blocking peptide.
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GTX02868 WB Image
WB analysis of extracts from mouse lung and rat kidney using GTX02868 c-Met (phospho Tyr1234) antibody. -/+ means absence or presence of N peptide(non-phospho peptide) and P peptide(phospho peptide).
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GTX02868 ELISA Image
ELISA analysis of phospho- and non phosho- immunogen peptide using GTX02868 c-Met (phospho Tyr1234) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml
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GTX02868 ICC/IF Image
ICC/IF analysis of PFA-fixed NIH-3T3 cells using GTX02868 c-Met (phospho Tyr1234) antibody.
Permeabilization : 0.1% Triton X-100
Dilution : 1:200
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REFERENCE
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REVIEW
There are currently no reviews for c-Met (phospho Tyr1234) antibody (GTX02868). Be the first to share your experience with this product.
