GeneTex
United States (US)

c-Myb antibody [N2C1], Internal

GTX102305_40457_WB_19011506_655.jpg

Cat No. GTX102305

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116

Predict Reactivity

Mouse, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human c-Myb. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cmyb antibody, c-myb antibody, c-myb_CDS antibody, efg antibody, MYB antibody, c-myb13A_CDS antibody, c-myb8B_CDS antibody, c-myb14A_CDS antibody, proto-oncogene c-Myb antibody, transcriptional activator Myb antibody, c-myb10A_CDS antibody, c-myb protein (140 AA) antibody, v-myb myeloblastosis viral oncogene homolog (avian) antibody

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor that is a member of the MYB family of transcription factor genes. The protein contains three domains, an N-terminal DNA-binding domain, a central transcriptional activation domain and a C-terminal domain involved in transcriptional repression. This protein plays an essential role in the regulation of hematopoiesis and may play a role in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102305_40457_WB_19011506_655.jpg

GTX102305 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
7.5% SDS PAGE
GTX102305 diluted at 1:1000

REVIEW

c-Myb antibody [N2C1], Internal Cat No. GTX102305

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 30-Mar-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : PC3 cells
Amount used : 20μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:3000, 4°C, 12Hr
SDS
Glycerol.pdf
Thimerosal.pdf