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c-Myb antibody [N2C1], Internal

Anti-c-Myb antibody [N2C1], Internal used in Western Blot (WB). GTX102305

Cat No. GTX102305

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human c-Myb. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MYB proto-oncogene, transcription factor , Cmyb , c-myb , c-myb_CDS , efg

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor that is a member of the MYB family of transcription factor genes. The protein contains three domains, an N-terminal DNA-binding domain, a central transcriptional activation domain and a C-terminal domain involved in transcriptional repression. This protein plays an essential role in the regulation of hematopoiesis and may play a role in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-c-Myb antibody [N2C1], Internal used in Western Blot (WB). GTX102305

GTX102305 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
7.5% SDS PAGE
GTX102305 diluted at 1:1000

REFERENCE

There are currently no references for c-Myb antibody [N2C1], Internal (GTX102305). Be the first to share your publications with this product.

REVIEW

c-Myb antibody [N2C1], Internal Cat No. GTX102305

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 30-Mar-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : PC3 cells
Amount used : 20μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:3000, 4°C, 12Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169