GeneTex
United States (US)

c-Myc antibody [1A6]

Overexpression
GTX84066_4110_WB_18121411_534.jpg
GTX84066_290_FACS_18121411_985.jpg
GTX84066_2289_IHC-P_19021902_565.jpg
GTX84066_2296_IHC-P_19021902_370.jpg
GTX84066_2295_IHC-P_19021902_913.jpg
GTX84066_2288_IHC-P_19021902_246.jpg
GTX84066_2298_IHC-P_19021902_144.jpg
GTX84066_2301_IHC-P_19021902_591.jpg
GTX84066_2291_IHC-P_19021902_313.jpg
GTX84066_2292_IHC-P_19021902_415.jpg
GTX84066_2294_IHC-P_19021902_987.jpg
GTX84066_2299_IHC-P_19021902_195.jpg
GTX84066_2300_IHC-P_19021902_506.jpg
GTX84066_2290_IHC-P_19021902_868.jpg
GTX84066_938_ICCIF_18121411_205.jpg
GTX84066_2297_IHC-P_19021902_177.jpg
GTX84066_3490_WB_19021902_488.jpg
GTX84066_2293_IHC-P_19021902_152.jpg
GTX84066_3489_WB_19021902_817.jpg

Cat No. GTX84066

Host Mouse
Clonality Monoclonal
Clone Name 1A6
Isotype IgG2b
Application WB, ICC/IF, IHC-P, FACS
Reactivity Human, Mouse, Monkey
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Specificity/Sensitivity

GTX84066 is specific for amino acids 1-454 of human c-Myc
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human Myc (NP_002458) produced in E.coli.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

v-myc myelocytomatosis viral oncogene homolog (avian) Antibody , MYC Antibody , MRTL Antibody , bHLHe39 Antibody , cmyc Antibody

Cellular Localization

Nucleus

Background

Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.

Database

Research Area

DATA IMAGES
GTX84066_4110_WB_18121411_534.jpg

GTX84066 WB Image

Overexpression

WB analysis of HEK293T cells transfected with c-Myc plasmid (Right) or empty vector (Left) for 48 hrs using GTX84066 c-Myc antibody [1A6].
Loading : 5 ug per lane

GTX84066_290_FACS_18121411_985.jpg

GTX84066 FACS Image

FACS analysis of HEK293T cells transfected with either c-Myc plasmid(Red) or empty vector control plasmid(Blue) using GTX84066 c-Myc antibody [1A6].

GTX84066_2289_IHC-P_19021902_565.jpg

GTX84066 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84066 c-Myc antibody [1A6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

GTX84066_2296_IHC-P_19021902_370.jpg

GTX84066 IHC-P Image

IHC-P analysis of human colon tissue using GTX84066 c-Myc antibody [1A6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

GTX84066_2295_IHC-P_19021902_913.jpg

GTX84066 IHC-P Image

IHC-P analysis of human breast tissue using GTX84066 c-Myc antibody [1A6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

GTX84066_2288_IHC-P_19021902_246.jpg

GTX84066 IHC-P Image

IHC-P analysis of human colon adenocarcinoma tissue using GTX84066 c-Myc antibody [1A6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

GTX84066_2298_IHC-P_19021902_144.jpg

GTX84066 IHC-P Image

IHC-P analysis of human liver tissue using GTX84066 c-Myc antibody [1A6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

GTX84066_2301_IHC-P_19021902_591.jpg

GTX84066 IHC-P Image

IHC-P analysis of human thyroid tissue using GTX84066 c-Myc antibody [1A6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

GTX84066_2291_IHC-P_19021902_313.jpg

GTX84066 IHC-P Image

IHC-P analysis of human liver carcinoma tissue using GTX84066 c-Myc antibody [1A6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

GTX84066_2292_IHC-P_19021902_415.jpg

GTX84066 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX84066 c-Myc antibody [1A6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

GTX84066_2294_IHC-P_19021902_987.jpg

GTX84066 IHC-P Image

IHC-P analysis of human thyroid carcinoma tissue using GTX84066 c-Myc antibody [1A6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

GTX84066_2299_IHC-P_19021902_195.jpg

GTX84066 IHC-P Image

IHC-P analysis of human lung tissue using GTX84066 c-Myc antibody [1A6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

GTX84066_2300_IHC-P_19021902_506.jpg

GTX84066 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84066 c-Myc antibody [1A6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

GTX84066_2290_IHC-P_19021902_868.jpg

GTX84066 IHC-P Image

IHC-P analysis of human kidney carcinoma tissue using GTX84066 c-Myc antibody [1A6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

GTX84066_938_ICCIF_18121411_205.jpg

GTX84066 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with c-Myc plasmid using GTX84066 c-Myc antibody [1A6].

GTX84066_2297_IHC-P_19021902_177.jpg

GTX84066 IHC-P Image

IHC-P analysis of human endometrium tissue using GTX84066 c-Myc antibody [1A6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

GTX84066_3490_WB_19021902_488.jpg

GTX84066 WB Image

WB analysis of various cell lines using GTX84066 c-Myc antibody [1A6].
Loading : 10 ug per lane
Dilution : 1:200

GTX84066_2293_IHC-P_19021902_152.jpg

GTX84066 IHC-P Image

IHC-P analysis of human pancreas carcinoma tissue using GTX84066 c-Myc antibody [1A6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

GTX84066_3489_WB_19021902_817.jpg

GTX84066 WB Image

WB analysis of various samples using GTX84066 c-Myc antibody [1A6].
Loading : 10 ug per lane
Dilution : 1:200

Package List Price ($)
$ 379