APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 1-2μg/ml for 30 minutes at RT |
| 1-2μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
Product Note
This antibody recognizes a transcription factor of 64-67kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This MAb shows no cross-reaction with v-myc.
We do not recommend use of this product for Mouse,Rat,Chicken samples.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide, corresponding to aa 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) from C-terminus of human c-myc, coupled to KLH.
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
MRTL , MYC , MYCC , bHLHe39 , cMyc , vmyc avian myelocytomatosis viral oncogene homolog , cmyc , MYC proto-oncogene, bHLH transcription factor
Cellular Localization
Nucleus,Nucleoplasm,Nucleolus
Background
This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
![Anti-c-Myc antibody [9E10.3] used in IHC (Paraffin sections) (IHC-P). GTX34610](/templates/default/js/lightbox/images/loading.gif "Anti-c-Myc antibody [9E10.3] used in IHC (Paraffin sections) (IHC-P). GTX34610")
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GTX34610 IHC-P Image
IHC-P analysis of human cervical carcinoma tissue using GTX34610 c-Myc antibody [9E10.3].
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REFERENCE
There are currently no references for c-Myc antibody [9E10.3] (GTX34610). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for c-Myc antibody [9E10.3] (GTX34610). Be the first to share your experience with this product.
![Anti-c-Myc antibody [9E10.3] used in IHC (Paraffin sections) (IHC-P). GTX34610](/upload/website/prouct_img/small/GTX34610/GTX34610_20200115_IHC-P_424_w_23060801_120.webp "Anti-c-Myc antibody [9E10.3] used in IHC (Paraffin sections) (IHC-P). GTX34610")
![Anti-c-Myc antibody [9E10.3] used in IHC (Paraffin sections) (IHC-P). GTX34610](/upload/website/prouct_img/normal/GTX34610/GTX34610_20200115_IHC-P_424_w_23060801_120.webp "Anti-c-Myc antibody [9E10.3] used in IHC (Paraffin sections) (IHC-P). GTX34610")