GeneTex
United States (US)

c-Myc antibody [9E10] (Biotin)

Cat No. GTX10825

Host Mouse
Clonality Monoclonal
Clone Name 9E10
Isotype IgG1
Application WB, IHC-P, IHC-Fr, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
ELISA 1/20-1/50

Note :

WB
This antibody recognizes c-myc under non-reducing conditions

Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Specificity/Sensitivity

This antibody recognizes the sequence EQKLISEEDL.
PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide, 1% BSA

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide sequence corresponding to the C-terminal region (residues 408-439) of human c-myc conjugated to keyhole limpet hemocyanin.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Biotin

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Myc Proto-Oncogene, Bhlh Transcription Factor,Mrtl,Mycc,Bhlhe39,C-Myc,Myc

Background

The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

REFERENCE
Package List Price ($)
$ 319