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c-Myc antibody [9E10]

Anti-c-Myc antibody [9E10] used in Western Blot (WB). GTX75953
Anti-c-Myc antibody [9E10] used in ELISA (ELISA). GTX75953

Cat No. GTX75953

Host

Mouse

Clonality

Monoclonal

Clone Name

9E10

Isotype

IgG1

Application

WB, ICC/IF, FACS, IP, ELISA

Reactivity

Human
Package
500 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Specificity/Sensitivity

This antibody recognizes the sequence EQKLISEEDL

PROPERTIES

Form

Liquid

Buffer

0.2 μM filtered in PBS pH 7

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide sequence corresponding to the C-terminal region (residues 408-439) of human c-myc conjugated to keyhole limpet hemocyanin.

Purification

Protein G purified
From tissue culture supernatant

Purity

>95% (Determined by SDS-PAGE)

Endotoxin

<0.002EU/μg (Determined by LAL gel clotting assay)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MRTL,MYC,MYCC,bHLHe39,cMyc,vmyc avian myelocytomatosis viral oncogene homolog,cmyc,MYC proto-oncogene, bHLH transcription factor

Cellular Localization

Nucleus,Nucleoplasm,Nucleolus

Background

This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-c-Myc antibody [9E10] used in Western Blot (WB). GTX75953

GTX75953 WB Image

WB analysis of HEK293 cell lysate using GTX75953 c-Myc antibody [9E10].

Anti-c-Myc antibody [9E10] used in ELISA (ELISA). GTX75953

GTX75953 ELISA Image

ELISA analysis of c-myc tagged recombinant protein using GTX75953 c-Myc antibody [9E10].

REFERENCE

REVIEW

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Package List Price ($)
$ 379