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c-Myc antibody [GT168]

Anti-c-Myc antibody [GT168] used in IHC (Paraffin sections) (IHC-P). GTX628459
Anti-c-Myc antibody [GT168] used in Immunoprecipitation (IP). GTX628459
Anti-c-Myc antibody [GT168] used in Flow cytometry (FACS). GTX628459
Anti-c-Myc antibody [GT168] used in Western Blot (WB). GTX628459
Anti-c-Myc antibody [GT168] used in Flow cytometry (FACS). GTX628459

Cat No. GTX628459

Host Mouse
Clonality Monoclonal
Clone Name GT168
Isotype IgG1
Application WB, IHC-P, FACS, IP
Reactivity Human
Package
200 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
FACS 1:50-1:200
IP 1:100-1:500
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

293T
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen used to generate this antibody corresponds to human c-Myc

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MYC proto-oncogene, bHLH transcription factor , MRTL , MYCC , bHLHe39 , c-Myc

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-c-Myc antibody [GT168] used in IHC (Paraffin sections) (IHC-P). GTX628459

GTX628459 IHC-P Image

c-Myc antibody [GT168] detects Myc protein at nucleus on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
c-Myc antibody [GT168] (GTX628459) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-c-Myc antibody [GT168] used in Immunoprecipitation (IP). GTX628459

GTX628459 IP Image

Immunoprecipitation of c-Myc protein from HeLa nuclear extracts using 5 μg of c-Myc antibody [GT168] (GTX628459).
Western blot analysis was performed using c-Myc antibody [GT168] (GTX628459).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-c-Myc antibody [GT168] used in Flow cytometry (FACS). GTX628459

GTX628459 FACS Image

c-Myc antibody [GT168] antibody (GTX628459) detects c-Myc protein by flow cytometry analysis.
Sample: NT2D1 cell fixed in 4% paraformaldehyde for 15 min on ice.
Black: Unlabelled sample was also used as a control.
Red: c-Myc antibody [GT168] antibody (GTX628459) dilution: 1:50.
Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-c-Myc antibody [GT168] used in Western Blot (WB). GTX628459

GTX628459 WB Image

c-Myc antibody [GT168] detects Myc protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
10 % SDS-PAGE
c-Myc antibody [GT168] (GTX628459) dilution: 1:500

Anti-c-Myc antibody [GT168] used in Flow cytometry (FACS). GTX628459

GTX628459 FACS Image

c-Myc antibody [GT168] detects C-MYC protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: c-Myc antibody [GT168] dilution: 1:50

REFERENCE
REVIEW

c-Myc antibody [GT168] Cat No. GTX628459

Rating ( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Flow cytometry(FACS) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 21-Mar-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : C-myc-tagged ACK1 expressing-transfected H1299-TO-p53 cells
Amount used : 60μg
Blocking : milk in TBST, 25°C, 1.5Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Lane Description : Lane A : H1299-TO-p53 cells, Lane B : C-myc-tagged ACK1 expressing-transfected H1299-TO-p53 cells
Date : Anonymous submitted on 16-May-2013
Score :
Application Tested : FACS
Sample Species : Hu
Sample : hES cells
Blocking : 2% serum, 4°C, 0.5Hr
Fixation : 70% ethanol
Primary Antibody : 1:50, 4°C, 0.5Hr
SDS
PBS.pdf
Package List Price ($)
$ 319