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c-Myc antibody [GT168]

Anti-c-Myc antibody [GT168] used in Western Blot (WB). GTX628459
Anti-c-Myc antibody [GT168] used in IHC (Paraffin sections) (IHC-P). GTX628459
Anti-c-Myc antibody [GT168] used in Immunoprecipitation (IP). GTX628459
Anti-c-Myc antibody [GT168] used in Flow cytometry (FACS). GTX628459
Anti-c-Myc antibody [GT168] used in Western Blot (WB). GTX628459
Anti-c-Myc antibody [GT168] used in Flow cytometry (FACS). GTX628459

Cat No. GTX628459

Host

Mouse

Clonality

Monoclonal

Clone Name

GT168

Isotype

IgG1

Application

WB, IHC-P, FACS, IP

Reactivity

Human
Package
200 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
FACS 1:50-1:200
IP 1:100-1:500
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

293T

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen used to generate this antibody corresponds to human c-Myc

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888034

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MYC proto-oncogene, bHLH transcription factor , MRTL , MYCC , bHLHe39 , c-Myc

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-c-Myc antibody [GT168] used in Western Blot (WB). GTX628459

GTX628459 WB Image

HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Myc antibody [GT168] (GTX628459) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-c-Myc antibody [GT168] used in IHC (Paraffin sections) (IHC-P). GTX628459

GTX628459 IHC-P Image

c-Myc antibody [GT168] detects Myc protein at nucleus on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
c-Myc antibody [GT168] (GTX628459) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-c-Myc antibody [GT168] used in Immunoprecipitation (IP). GTX628459

GTX628459 IP Image

Immunoprecipitation of c-Myc protein from HeLa nuclear extracts using 5 μg of c-Myc antibody [GT168] (GTX628459).
Western blot analysis was performed using c-Myc antibody [GT168] (GTX628459).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-c-Myc antibody [GT168] used in Flow cytometry (FACS). GTX628459

GTX628459 FACS Image

c-Myc antibody [GT168] antibody (GTX628459) detects c-Myc protein by flow cytometry analysis.
Sample: NT2D1 cell fixed in 4% paraformaldehyde for 15 min on ice.
Black: Unlabelled sample was also used as a control.
Red: c-Myc antibody [GT168] antibody (GTX628459) dilution: 1:50.
Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-c-Myc antibody [GT168] used in Western Blot (WB). GTX628459

GTX628459 WB Image

c-Myc antibody [GT168] detects Myc protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
10 % SDS-PAGE
c-Myc antibody [GT168] (GTX628459) dilution: 1:500

Anti-c-Myc antibody [GT168] used in Flow cytometry (FACS). GTX628459

GTX628459 FACS Image

c-Myc antibody [GT168] detects C-MYC protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: c-Myc antibody [GT168] dilution: 1:50

REFERENCE

REVIEW

c-Myc antibody [GT168] Cat No. GTX628459

Rating ( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Flow cytometry(FACS) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 21-Mar-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : C-myc-tagged ACK1 expressing-transfected H1299-TO-p53 cells
Amount used : 60μg
Blocking : milk in TBST, 25°C, 1.5Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Lane Description : Lane A : H1299-TO-p53 cells, Lane B : C-myc-tagged ACK1 expressing-transfected H1299-TO-p53 cells
Date : Anonymous submitted on 16-May-2013
Score :
Application Tested : FACS
Sample Species : Hu
Sample : hES cells
Blocking : 2% serum, 4°C, 0.5Hr
Fixation : 70% ethanol
Primary Antibody : 1:50, 4°C, 0.5Hr
SDS
PBS.pdf