GeneTex
United States (US)

c-Myc antibody

KO/KD Orthogonal
GTX103436_40884_ChIP_19011506_523.jpg
GTX103436_40884_IP_19011506_747.jpg
GTX103436_40884_20180202_WB_KO_watermark_19011506_422.jpg
GTX103436_40884_20150601_IFA_19011506_675.jpg
GTX103436_40884_WB_M_19011506_979.jpg
GTX103436_40884_20151015_WB_Fraction_19011506_426.jpg
GTX103436_40884_WB_sensitivity_19011506_845.jpg

Cat No. GTX103436

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ChIP assay
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

293T , NIH-3T3 , Raw264.7 , HeLa , HeLa nuclear extract

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Cat, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human c-Myc. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MRTL antibody, bHLHe39 antibody, c-Myc antibody, MYC antibody, avian myelocytomatosis viral oncogene homolog antibody, myc-related translation/localization regulatory factor antibody, class E basic helix-loop-helix protein 39 antibody, transcription factor p64 antibody, proto-oncogene c-Myc antibody, myc proto-oncogene protein antibody, v-myc avian myelocytomatosis viral oncogene homolog antibody, v-myc myelocytomatosis viral oncogene homolog (avian) antibody

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103436_40884_ChIP_19011506_523.jpg

GTX103436 ChIP assay Image

c-Myc antibody immunoprecipitates c-Myc protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of c-Myc antibody (GTX103436) The precipitated DNA was detected by PCR with primer set targeting to mSHMT promoter.

GTX103436_40884_IP_19011506_747.jpg

GTX103436 IP Image

c-Myc antibody immunoprecipitates c-Myc protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 20 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of c-Myc protein by 2.5 μg of c-Myc antibody (GTX103436) 10% SDS-PAGE The immunoprecipitated c-Myc protein was detected by c-Myc antibody (GTX103436) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX103436_40884_20180202_WB_KO_watermark_19011506_422.jpg

GTX103436 WB Image

KO/KD

Wild-type (WT) and c-Myc knockout (KO) 293T cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Myc antibody (GTX103436) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
CRISPR knockout cell lines generated by www.edigene.com

GTX103436_40884_20150601_IFA_19011506_675.jpg

GTX103436 ICC/IF Image

c-Myc antibody detects c-Myc protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: c-Myc protein stained by c-Myc antibody (GTX103436) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX103436_40884_WB_M_19011506_979.jpg

GTX103436 WB Image

Sample (30 µg of whole cell lysate) 
  A: NIH-3T3
  B: Raw264.7
  10% SDS PAGE 
  GTX103436 diluted at 1:1000
   
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103436_40884_20151015_WB_Fraction_19011506_426.jpg

GTX103436 WB Image

Orthogonal

c-Myc antibody detects c-Myc protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with c-Myc antibody (GTX103436) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103436_40884_WB_sensitivity_19011506_845.jpg

GTX103436 WB Image

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
10% SDS PAGE
GTX103436 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169