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c-Myc antibody

Anti-c-Myc antibody used in ChIP assay (ChIP assay). GTX103436
Anti-c-Myc antibody used in Immunoprecipitation (IP). GTX103436
Anti-c-Myc antibody used in Western Blot (WB). GTX103436
Anti-c-Myc antibody used in Western Blot (WB). GTX103436
Anti-c-Myc antibody used in Western Blot (WB). GTX103436
Anti-c-Myc antibody used in Western Blot (WB). GTX103436

Cat. No. GTX103436

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP, ChIP assay

Reactivity

Human, Mouse, Xenopus laevis
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC-Fr Assay dependent
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

293T

Product Note

This antibody can detect the endogenous c-Myc, but it is unable to recognize the c-Myc tag.

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Cat, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human c-Myc. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11162914

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MYC proto-oncogene, bHLH transcription factor , MRTL , MYCC , bHLHe39 , c-Myc

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-c-Myc antibody used in ChIP assay (ChIP assay). GTX103436

GTX103436 ChIP assay Image

c-Myc antibody immunoprecipitates c-Myc protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of c-Myc antibody (GTX103436) The precipitated DNA was detected by PCR with primer set targeting to mSHMT promoter.

Anti-c-Myc antibody used in Immunoprecipitation (IP). GTX103436

GTX103436 IP Image

c-Myc antibody immunoprecipitates c-Myc protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 20 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of c-Myc protein by 2.5 μg of c-Myc antibody (GTX103436) 10% SDS-PAGE The immunoprecipitated c-Myc protein was detected by c-Myc antibody (GTX103436) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-c-Myc antibody used in Western Blot (WB). GTX103436

GTX103436 WB Image

HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Myc antibody (GTX103436) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-c-Myc antibody used in Western Blot (WB). GTX103436

GTX103436 WB Image

Wild-type (WT) and MYC knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Myc antibody (GTX103436) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-c-Myc antibody used in Western Blot (WB). GTX103436

GTX103436 WB Image

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
10% SDS PAGE
GTX103436 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-c-Myc antibody used in Western Blot (WB). GTX103436

GTX103436 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Myc antibody (GTX103436) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

c-Myc antibody Cat. No. GTX103436

Rating ( Average 5 based on 6 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 1 users reviews)
ChIP assay(ChIP assay) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncogenesis in 2020.PMID: 32350243
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018.PMID: 29875371
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Front Mol Neurosci in 2017.PMID: 29234272
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Front Mol Neurosci in 2017.PMID: 29234272
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015. PMID: 26226629
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ChIP assay
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Pharmacol Res Perspect in 2018. PMID: 30455960
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169