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c-Myc antibody

KO/KD Orthogonal Overexpression
Anti-c-Myc antibody used in Western Blot (WB). GTX103436
Anti-c-Myc antibody used in ChIP assay (ChIP assay). GTX103436
Anti-c-Myc antibody used in Immunoprecipitation (IP). GTX103436
Anti-c-Myc antibody used in Western Blot (WB). GTX103436
Anti-c-Myc antibody used in Western Blot (WB). GTX103436
Anti-c-Myc antibody used in Western Blot (WB). GTX103436

Cat. No. GTX103436

Reference 27
6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, ChIP assay

Reactivity

 Human, Mouse, Xenopus laevis
Package
100 μl ($399),
25 μl ($169)
See all c-Myc products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC-Fr Assay dependent
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Cat, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human c-Myc. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11162914

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MYC proto-oncogene, bHLH transcription factor , MRTL , MYCC , bHLHe39 , c-Myc

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-c-Myc antibody used in Western Blot (WB). GTX103436

GTX103436 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Myc antibody (GTX103436) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-c-Myc antibody used in ChIP assay (ChIP assay). GTX103436

GTX103436 ChIP assay Image

c-Myc antibody immunoprecipitates c-Myc protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of c-Myc antibody (GTX103436) The precipitated DNA was detected by PCR with primer set targeting to mSHMT promoter.

Anti-c-Myc antibody used in Immunoprecipitation (IP). GTX103436

GTX103436 IP Image

c-Myc antibody immunoprecipitates c-Myc protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 20 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of c-Myc protein by 2.5 μg of c-Myc antibody (GTX103436) 10% SDS-PAGE The immunoprecipitated c-Myc protein was detected by c-Myc antibody (GTX103436) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-c-Myc antibody used in Western Blot (WB). GTX103436

GTX103436 WB Image

KO/KD

Wild-type (WT) and MYC knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Myc antibody (GTX103436) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-c-Myc antibody used in Western Blot (WB). GTX103436

GTX103436 WB Image

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
10% SDS PAGE
GTX103436 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-c-Myc antibody used in Western Blot (WB). GTX103436

GTX103436 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Myc antibody (GTX103436) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Mushtaq I et al. Front Oncol 2022; 12 : 919351 Telomere Attrition With Concomitant hTERT Overexpression Involved in the Progression of Gastric Cancer May Have Prognostic and Clinical Implications in High-Risk Population Group From North India.
Application : IHC-P
Reactivity : Human
Huang CY et al. Life (Basel) 2021; 11 (9) Calcitriol Suppresses Warburg Effect and Cell Growth in Human Colorectal Cancer Cells.
Application : WB
Reactivity : Human
Hsu WJ et al. J Pers Med 2021; 11 (10) PRMT1 Confers Resistance to Olaparib via Modulating MYC Signaling in Triple-Negative Breast Cancer.
Application : WB
Reactivity : Human
Wu MJ et al. Theranostics 2021; 11 (16) : 7779-7796 Targeting KDM4B that coactivates c-Myc-regulated metabolism to suppress tumor growth in castration-resistant prostate cancer.
Application : IHC-P
Qiu S et al. Front Microbiol 2021; 12 : 690988 Downregulation of Light-Harvesting Complex II Induces ROS-Mediated Defense Against Turnip Mosaic Virus Infection in Nicotiana benthamiana.
Application : WB
Show More +
Submit a Reference

REVIEW

c-Myc antibody Cat. No. GTX103436

Rating
( Average 5 based on 6 users reviews)
Application
Western Blot(WB)
( Average 5 based on 3 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
IHC (Frozen sections)(IHC-Fr)
( Average 5 based on 1 users reviews)
ChIP assay(ChIP assay)
( Average 5 based on 1 users reviews)
Anti-c-Myc antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103436
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncogenesis in 2020.PMID: 32350243
Anti-c-Myc antibody used in Western Blot (WB). GTX103436
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018.PMID: 29875371
Anti-c-Myc antibody used in Western Blot (WB). GTX103436
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Front Mol Neurosci in 2017.PMID: 29234272
Anti-c-Myc antibody used in IHC (Frozen sections) (IHC-Fr). GTX103436
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Front Mol Neurosci in 2017.PMID: 29234272
Anti-c-Myc antibody used in Western Blot (WB). GTX103436
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015. PMID: 26226629
Anti-c-Myc antibody used in ChIP assay (ChIP assay). GTX103436
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ChIP assay
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Pharmacol Res Perspect in 2018. PMID: 30455960
Show More +
Submit a Review
Application Reference
Mushtaq I et al. Front Oncol 2022; 12:919351 Telomere Attrition With Concomitant hTERT Overexpression Involved in the Progression of Gastric Cancer May Have Prognostic and Clinical Implications in High-Risk Population Group From North India.
Application : IHC-P
Reactivity : Human
Huang CY et al. Life (Basel) 2021; 11 (9) Calcitriol Suppresses Warburg Effect and Cell Growth in Human Colorectal Cancer Cells.
Application : WB
Reactivity : Human
Hsu WJ et al. J Pers Med 2021; 11 (10) PRMT1 Confers Resistance to Olaparib via Modulating MYC Signaling in Triple-Negative Breast Cancer.
Application : WB
Reactivity : Human
Wu MJ et al. Theranostics 2021; 11 (16):7779-7796 Targeting KDM4B that coactivates c-Myc-regulated metabolism to suppress tumor growth in castration-resistant prostate cancer.
Application : IHC-P
Qiu S et al. Front Microbiol 2021; 12:690988 Downregulation of Light-Harvesting Complex II Induces ROS-Mediated Defense Against Turnip Mosaic Virus Infection in Nicotiana benthamiana.
Application : WB
Lee CC et al. Life Sci 2021; 277:119438 A regulatory loop among CD276, miR-29c-3p, and Myc exists in cancer cells against natural killer cell cytotoxicity.
Application : WB
Colozza G et al. MicroPubl Biol 2021; 2021 Purified Bighead protein efficiently promotes head development in the South African clawed frog, Xenopus laevis.
Reactivity : Xenopus laevis
Ge J et al. J Exp Clin Cancer Res 2021; 40 (1):59 USP16 regulates castration-resistant prostate cancer cell proliferation by deubiquitinating and stablizing c-Myc.
Application : WB
Reactivity : Human
Chen J et al. Oncogenesis 2020; 9 (4):42 ACSL4 promotes hepatocellular carcinoma progression via c-Myc stability mediated by ERK/FBW7/c-Myc axis.
Reactivity : Human
Classen J et al. Sci Rep 2020; 10 (1):3181 A Munc18-1 mutant mimicking phosphorylation by Down Syndrome-related kinase Dyrk1a supports normal synaptic transmission and promotes recovery after intense activity.
Application : WB
Zhang J et al. Artif Cells Nanomed Biotechnol 2020; 48 (1):479-487 Inhibition of Wnt signalling pathway by XAV939 enhances radiosensitivity in human cervical cancer HeLa cells.
Application : WB
Reactivity : Human
Davuluri G et al. Mol Cell Biol 2019; 39 (16) Impaired Ribosomal Biogenesis by Noncanonical Degradation of β-Catenin during Hyperammonemia.
Fang Y et al. Nat Commun 2019; 10 (1):3981 CD36 inhibits 帣-catenin/c-myc-mediated glycolysis through ubiquitination of GPC4 to repress colorectal tumorigenesis.
Application : IHC-P
Reactivity : Human
Chen YH et al. Sci Rep 2019; 9 (1):9919 Mitochondrial Akt Signaling Modulated Reprogramming of Somatic Cells.
Reactivity : Human
Seira N et al. Pharmacol Res Perspect 2018; 6 (6):e00441 Cellular density-dependent increases in HIF-1α compete with c-Myc to down-regulate human EP4 receptor promoter activity through Sp-1-binding region.
Application : IP
Reactivity : Human
Zhang K et al. Mol Cancer 2018; 17 (1):153 Targeting histone methyltransferase G9a inhibits growth and Wnt signaling pathway by epigenetically regulating HP1α and APC2 gene expression in non-small cell lung cancer.
Application : WB
Reactivity : Human
Lin CK et al. Sci Rep 2018; 8 (1):8676 Lobohedleolide suppresses hepatitis C virus replication via JNK/c-Jun-C/EBP-mediated down-regulation of cyclooxygenase-2 expression.
Application : WB
Reactivity : Human
Chen KC et al. Journal of Food and Drug Analysis 2018; (Epub) Flavonoids Luteolin and Quercetin Inhibit RPS19 and contributes to metastasis of cancer cells through c-Myc reduction.
Application : WB
Reactivity : Human
Yang JS et al. Oncol Rep 2018; 39 (1):383-391 ITR-284 modulates cell differentiation in human chronic myelogenous leukemia K562 cells.
Ma YX et al. Front Mol Neurosci 2017; 10:391 Differential Roles of Glycogen Synthase Kinase 3 Subtypes Alpha and Beta in Cortical Development.
Application : WB
Lee YC et al. Int J Mol Sci 2017; 18 (9) Hsp90α Mediates BMI1 Expression in Breast Cancer Stem/Progenitor Cells through Facilitating Nuclear Translocation of c-Myc and EZH2.
Application : WB
Reactivity : Human
Lin HC et al. J Agric Food Chem 2017; 65 (1):51-59 Antroquinonol, a Ubiquinone Derivative from the Mushroom Antrodia camphorata, Inhibits Colon Cancer Stem Cell-like Properties: Insights into the Molecular Mechanism and Inhibitory Targets.
Application : WB
Reactivity : Human
Chung IH et al. Oncotarget 2016; 7 (16):22448-59 ChIP-on-chip analysis of thyroid hormone-regulated genes and their physiological significance.
Application : WB
Reactivity : Human
Fan F et al. Biomed Pharmacother 2016; 84:1858-1864 Nuclear PKM2 expression, an independent risk factor for ER after curative resection of hepatocellular carcinoma.
Huang CC et al. Oncotarget 2015; 6 (11):8698-708 Paxillin promotes colorectal tumor invasion and poor patient outcomes via ERK-mediated stabilization of Bcl-2 protein by phosphorylation at Serine 87.
Application : WB, IHC-P
Reactivity : Human
Wu MH et al. PLoS One 2015; 10 (7):e0134122 Macrophage Infiltration Induces Gastric Cancer Invasiveness by Activating the β-Catenin Pathway.
Fu Y et al. Med Oncol 2015; 32 (8):215 FBXW7 overexpression suppresses renal cancer cell proliferation and induces apoptosis.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169