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c-Myc antibody

Cat. No. GTX20056

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA, ChIP assay

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all c-Myc products

APPLICATION

Application Note

Suggested working dilutions: Immunoprecipitation: 2 μg/mg protein lysate Western blotting: 1:500-1:1000. Optimal dilutions/concentrations should be determined by the end user

Calculated MW

49 kDa. ( Note )

Positive Control

HL-60 cells or breast tumors.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: AEEQKLISEEDL conjugated to KLH, corresponding to amino acids 408-420 of Human c-Myc.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_368678

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MYC proto-oncogene, bHLH transcription factor , MRTL , MYCC , bHLHe39 , c-Myc

Cellular Localization

nucleoplasm , Nucleolus

Background

This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]

Database

Research Area

REFERENCE

Application Reference

Bailey CC et al. J Biol Chem 2013; 288 (45) : 32184-93 Interferon-induced transmembrane protein 3 is a type ii transmembrane protein.
Application : ICC/IF
Sbia M et al. J Biol Chem 2008; 283 (17) : 11135-45 Regulation of the yeast Ace2 transcription factor during the cell cycle.
Application : WB
Roberts DN et al. Mol Cell 2006; 22 (5) : 633-44 Dephosphorylation and genome-wide association of Maf1 with Pol III-transcribed genes during repression.
Application : IP
Bean JM et al. Genetics 2005; 171 (1) : 49-61 High functional overlap between MluI cell-cycle box binding factor and Swi4/6 cell-cycle box binding factor in the G1/S transcriptional program in Saccharomyces cerevisiae.
Application : ChIP assay
Wilmes GM et al. Genes Dev 2004; 18 (9) : 981-91 Interaction of the S-phase cyclin Clb5 with an "RXL" docking sequence in the initiator protein Orc6 provides an origin-localized replication control switch.
Application : ChIP assay
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Package List Price ($)
$ 399
Custom Conjugations