Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Myc (phospho Ser62) antibody [GT1295] (GTX03207) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Myc (phospho Ser62) antibody [GT1295] (GTX03207) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

WB analysis of NIH-3T3 cells were treated by Forskolin (30 uM) at 37ºC for 30 minutes after serum-starvation overnight using GTX03207 c-Myc (phospho Ser62) antibody [GT1295].
Dilution : 1:1000
Loading : 25μg per lane

WB analysis of 293T cells were treated by Forskolin (10 uM) at 37ºC for 30 minutes after serum-starvation overnight using GTX03207 c-Myc (phospho Ser62) antibody [GT1295].
Dilution : 1:1000
Loading : 25μg per lane