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c-Myc (phospho Ser62) antibody [GT1295]

Recombinant antibody Orthogonal
Anti-c-Myc (phospho Ser62) antibody [GT1295] used in Western Blot (WB). GTX03207
Anti-c-Myc (phospho Ser62) antibody [GT1295] used in Western Blot (WB). GTX03207
Anti-c-Myc (phospho Ser62) antibody [GT1295] used in Western Blot (WB). GTX03207
Anti-c-Myc (phospho Ser62) antibody [GT1295] used in Western Blot (WB). GTX03207

Cat. No. GTX03207

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1295

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($419)
See all c-Myc products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S62 of human c-Myc.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MYC proto-oncogene, bHLH transcription factor , MRTL , MYCC , bHLHe39 , c-Myc

Cellular Localization

nucleoplasm , Nucleolus

Background

This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-c-Myc (phospho Ser62) antibody [GT1295] used in Western Blot (WB). GTX03207

GTX03207 WB Image

Orthogonal

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Myc (phospho Ser62) antibody [GT1295] (GTX03207) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-c-Myc (phospho Ser62) antibody [GT1295] used in Western Blot (WB). GTX03207

GTX03207 WB Image

Orthogonal

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Myc (phospho Ser62) antibody [GT1295] (GTX03207) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-c-Myc (phospho Ser62) antibody [GT1295] used in Western Blot (WB). GTX03207

GTX03207 WB Image

Orthogonal

WB analysis of 293T cells were treated by Forskolin (10 uM) at 37ºC for 30 minutes after serum-starvation overnight using GTX03207 c-Myc (phospho Ser62) antibody [GT1295].
Dilution : 1:1000
Loading : 25μg per lane

Anti-c-Myc (phospho Ser62) antibody [GT1295] used in Western Blot (WB). GTX03207

GTX03207 WB Image

Orthogonal

WB analysis of NIH-3T3 cells were treated by Forskolin (30 uM) at 37ºC for 30 minutes after serum-starvation overnight using GTX03207 c-Myc (phospho Ser62) antibody [GT1295].
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 419