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c-Myc (phospho Thr358) antibody

Anti-c-Myc (phospho Thr358) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50112
Anti-c-Myc (phospho Thr358) antibody used in Western Blot (WB). GTX50112
Anti-c-Myc (phospho Thr358) antibody used in IHC (Paraffin sections) (IHC-P). GTX50112

Cat No. GTX50112

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 358 (R-R-T(p)-H-N) derived from human c-Myc.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MYC proto-oncogene, bHLH transcription factor , MRTL , MYCC , bHLHe39 , c-Myc

Cellular Localization

nucleoplasm,Nucleolus

Background

This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-c-Myc (phospho Thr358) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50112

GTX50112 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50112 c-Myc (phospho Thr358) antibody.

Anti-c-Myc (phospho Thr358) antibody used in Western Blot (WB). GTX50112

GTX50112 WB Image

WB analysis of extracts from HT-29 cells untreated (lane 1) or treated with UV (lane 2) using GTX50112 c-Myc (phospho Thr358) antibody.

Anti-c-Myc (phospho Thr358) antibody used in IHC (Paraffin sections) (IHC-P). GTX50112

GTX50112 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50112 c-Myc (phospho Thr358) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

There are currently no references for c-Myc (phospho Thr358) antibody (GTX50112). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for c-Myc (phospho Thr358) antibody (GTX50112). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf