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c-Myc (phospho Thr358) antibody

Anti-c-Myc (phospho Thr358) antibody used in IHC (Paraffin sections) (IHC-P). GTX79006
Anti-c-Myc (phospho Thr358) antibody used in Western Blot (WB). GTX79006

Cat. No. GTX79006

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human Myc around the phosphorylation site of threonine 358 (R-R-Tp-H-N).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11167787

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MRTL,MYC,MYCC,bHLHe39,cMyc,vmyc avian myelocytomatosis viral oncogene homolog,cmyc,MYC proto-oncogene, bHLH transcription factor

Cellular Localization

Nucleus,Nucleoplasm,Nucleolus

Background

This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-c-Myc (phospho Thr358) antibody used in IHC (Paraffin sections) (IHC-P). GTX79006

GTX79006 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX79006 c-Myc (phospho Thr358) antibody.

Anti-c-Myc (phospho Thr358) antibody used in Western Blot (WB). GTX79006

GTX79006 WB Image

WB analysis of HT-29 cells treated with UV (20min) lysate using GTX79006 c-Myc (phospho Thr358) antibody (Lane 3 and 4) and c-Myc antibody (Lane 1 and 2).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399