APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000 |
Not tested in other applications.
Calculated MW
Observed MW
57 kDa. The observed M.W. is based on the following publication. PMID: 24444383
PROPERTIES
Form
Liquid
Buffer
1x TBS (pH7.4), 1% BSA, 40% Glycerol
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
MYC proto-oncogene, bHLH transcription factor , MRTL , MYC , MYCC , bHLHe39 , cMyc , vmyc avian myelocytomatosis viral oncogene homolog , cmyc
Cellular Localization
Nucleus,Nucleoplasm,Nucleolus
Background
This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
![Anti-c-Myc (phospho Thr58/Ser62) antibody [SZ02-06] used in Western Blot (WB). GTX01137](/templates/default/js/lightbox/images/loading.gif "Anti-c-Myc (phospho Thr58/Ser62) antibody [SZ02-06] used in Western Blot (WB). GTX01137")
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GTX01137 WB Image
Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Myc (phospho Thr58/Ser62) antibody [SZ02-06] (GTX01137) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 24444383
|
REFERENCE
There are currently no references for c-Myc (phospho Thr58/Ser62) antibody [SZ02-06] (GTX01137). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for c-Myc (phospho Thr58/Ser62) antibody [SZ02-06] (GTX01137). Be the first to share your experience with this product.
![Anti-c-Myc (phospho Thr58/Ser62) antibody [SZ02-06] used in Western Blot (WB). GTX01137](/upload/website/prouct_img/small/GTX01137/GTX01137_HK1219_20200228_WB_treatment_MG132_w_23053121_696.webp "Anti-c-Myc (phospho Thr58/Ser62) antibody [SZ02-06] used in Western Blot (WB). GTX01137")
![Anti-c-Myc (phospho Thr58/Ser62) antibody [SZ02-06] used in Western Blot (WB). GTX01137](/upload/website/prouct_img/normal/GTX01137/GTX01137_HK1219_20200228_WB_treatment_MG132_w_23053121_696.webp "Anti-c-Myc (phospho Thr58/Ser62) antibody [SZ02-06] used in Western Blot (WB). GTX01137")