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c-Rel antibody [N1N3]

Anti-c-Rel antibody [N1N3] used in Immunoprecipitation (IP). GTX113264
Anti-c-Rel antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113264

Cat. No. GTX113264

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all c-Rel products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human c-Rel. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951679

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

REL proto-oncogene, NF-kB subunit , C-Rel

Cellular Localization

Nucleus

Background

The REL gene encodes c-Rel, a transcription factor that is a member of the Rel/NFKB family, which also includes RELA (MIM 164014), RELB (604758), NFKB1 (MIM 164011), and NFKB2 (MIM 164012). These proteins are related through a highly conserved N-terminal region termed the 'Rel domain,' which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFKB inhibitor (MIM 164008) (Belguise and Sonenshein, 2007 [PubMed 18037997]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-c-Rel antibody [N1N3] used in Immunoprecipitation (IP). GTX113264

GTX113264 IP Image

Immunoprecipitation of c-Rel protein from Raji whole cell extracts using 5 μg of c-Rel antibody [N1N3] (GTX113264).
Western blot analysis was performed using c-Rel antibody [N1N3] (GTX113264) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-c-Rel antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113264

GTX113264 IHC-P Image

c-Rel antibody [N1N3] detects REL protein at nucleus on human skin by immunohistochemical analysis.
Sample: Paraffin-embedded skin.
c-Rel antibody [N1N3] (GTX113264) dilution: 1:100.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Xu X et al. J Exp Clin Cancer Res 2021; 40 (1) : 253 Phosphorylation of NF-κBp65 drives inflammation-mediated hepatocellular carcinogenesis and is a novel therapeutic target.
Reactivity : Human
Yoshitomo Shiroma et al. Int J Mol Sci 2020; 21 (23) Identification of a Selective RelA Inhibitor Based on DSE-FRET Screening Methods.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169