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c-Yes antibody

Anti-c-Yes antibody used in Western Blot (WB). GTX100616
Anti-c-Yes antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100616
Anti-c-Yes antibody used in Western Blot (WB). GTX100616
Anti-c-Yes antibody used in Western Blot (WB). GTX100616

Cat No. GTX100616

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-Fr
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

A431 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , PC-12 , Rat2

Predict Reactivity

Zebrafish, Bovine, Dog, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human c-Yes. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

YES proto-oncogene 1, Src family tyrosine kinase,HsT441,P61-YES,Yes,c-yes

Background

This gene is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-c-Yes antibody used in Western Blot (WB). GTX100616

GTX100616 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with c-Yes antibody (GTX100616) diluted at 1:500.

Anti-c-Yes antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100616

GTX100616 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using c-Yes(GTX100616) antibody at 1:200 dilution.

Anti-c-Yes antibody used in Western Blot (WB). GTX100616

GTX100616 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX100616 diluted at 1:1000

Anti-c-Yes antibody used in Western Blot (WB). GTX100616

GTX100616 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with c-Yes antibody (GTX100616) diluted at 1:500.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169