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c-Yes antibody

Anti-c-Yes antibody used in Western Blot (WB). GTX100616
Anti-c-Yes antibody used in Western Blot (WB). GTX100616
Anti-c-Yes antibody used in Western Blot (WB). GTX100616
Anti-c-Yes antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100616

Cat. No. GTX100616

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all c-Yes products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

A431 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , PC-12 , Rat2

Predict Reactivity

Zebrafish, Bovine, Dog, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human c-Yes. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240675

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

YES proto-oncogene 1, Src family tyrosine kinase , HsT441 , P61-YES , Yes , c-yes

Background

This gene is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-c-Yes antibody used in Western Blot (WB). GTX100616

GTX100616 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with c-Yes antibody (GTX100616) diluted at 1:500.

Anti-c-Yes antibody used in Western Blot (WB). GTX100616

GTX100616 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with c-Yes antibody (GTX100616) diluted at 1:500.

Anti-c-Yes antibody used in Western Blot (WB). GTX100616

GTX100616 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX100616 diluted at 1:1000

Anti-c-Yes antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100616

GTX100616 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using c-Yes(GTX100616) antibody at 1:200 dilution.

REFERENCE

Application Reference

Lee JH et al. EBioMedicine 2018; (Epub) HIV Activates the Tyrosine Kinase Hck to Secrete ADAM Protease-Containing Extracellular Vesicles.
Application : IHC-Fr
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169