APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.25-0.5 μg/mL |
| 5 μg/mL |
| 2-5 μg/mL |
| 1-3 μg/1x10⁶ cells |
| 0.1-0.5 μg/mL |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄
Preservative
No preservatives
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
E.coli-derived human cGKI/PRKG1 recombinant protein (Position: S2-Q44).
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein kinase cGMP-dependent 1 , AAT8 , PKG , PKG1 , PRKG1B , PRKGR1B , cGK , cGK 1 , cGK1 , cGKI , cGKI-BETA , cGKI-alpha
Cellular Localization
Cytoplasm
Background
Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity. [provided by RefSeq, Sep 2011]
Database
Research Area
DATA IMAGES
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GTX04678 IHC-P Image
IHC-P analysis of mouse lung tissue using GTX04678 cGK1 antibody.
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 2 μg/mL
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GTX04678 IHC-P Image
IHC-P analysis of human breast cancer tissue using GTX04678 cGK1 antibody.
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 2 μg/mL
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GTX04678 FACS Image
FACS analysis of RH35 cells using GTX04678 cGK1 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample
Dilution : 1μg/1x10⁶ cells
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GTX04678 IHC-P Image
IHC-P analysis of rat lung tissue using GTX04678 cGK1 antibody.
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 2 μg/mL
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GTX04678 FACS Image
FACS analysis of U251 cells using GTX04678 cGK1 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample
Dilution : 1μg/1x10⁶ cells
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GTX04678 WB Image
WB analysis of various samples using GTX04678 cGK1 antibody.
Loading : 50 μg
Dilution : 0.5 μg/mL
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GTX04678 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX04678 cGK1 antibody.
Green : Primary antibody
Blue : DAPI
Antigen retrieval : Enzyme antigen retrieval
Dilution : 5 μg/mL
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REFERENCE
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REVIEW
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